Wei Y X, Sun B, Xiao L, Shi B Y
Organ Transplantation Institute, Beijing Key Laboratory of Organ Transplantation and Immune Regulation, 309th Hospital of the Chinese People's Liberation Army, Beijing, China.
Organ Transplantation Institute, Beijing Key Laboratory of Organ Transplantation and Immune Regulation, 309th Hospital of the Chinese People's Liberation Army, Beijing, China.
Transplant Proc. 2018 Dec;50(10):3906-3910. doi: 10.1016/j.transproceed.2018.04.066. Epub 2018 May 4.
Extracorporeal photopheresis (ECP) represents an alternative to immunosuppression as a means of reducing rejection after thoracic organ transplantation. The mechanism by which ECP exerts its protective effects, until now, has remained elusive. Infusion of ECP-treated splenic lymphocytes (PUVA-SP) can induce CD4CD25Foxp3 regulatory T cells. However, the regulatory effect of PUVA-SP on B cells remains poorly understood. In the present study, we measured IL-10 secretion from CD19 B cells of peripheral blood mononuclear cells. Our results demonstrate that infusion of PUVA-SP (PUVA-BSP from BALB/c or PUVA-CSP from C57BL/6 mice), in the absence of an immunosuppressant, significantly promotes skin allograft survival. This effect was associated with upregulation of circulating regulatory B cells exhibiting preferential IL-10 secretion and a shift of cytokine profile from helper T cell type 1 to helper T cell type 2. Our results suggest that effective treatments involving infusion of PUVA-SP is likely related not only to the modulation of T cell and regulatory T cell functions but also to the function of B cell and regulatory B cells.
体外光化学疗法（ECP）作为一种减少胸段器官移植后排斥反应的手段，是免疫抑制的一种替代方法。迄今为止，ECP发挥其保护作用的机制仍不清楚。输注经ECP处理的脾淋巴细胞（PUVA-SP）可诱导CD4CD25Foxp3调节性T细胞。然而，PUVA-SP对B细胞的调节作用仍知之甚少。在本研究中，我们检测了外周血单个核细胞中CD19 B细胞分泌白细胞介素-10（IL-10）的情况。我们的结果表明，在不使用免疫抑制剂的情况下，输注PUVA-SP（来自BALB/c小鼠的PUVA-BSP或来自C57BL/6小鼠的PUVA-CSP）可显著提高皮肤同种异体移植的存活率。这种效应与循环调节性B细胞的上调有关，这些调节性B细胞表现出优先分泌IL-10的特性，并且细胞因子谱从辅助性T细胞1型向辅助性T细胞2型转变。我们的结果表明，涉及输注PUVA-SP的有效治疗可能不仅与T细胞和调节性T细胞功能的调节有关，还与B细胞和调节性B细胞的功能有关。
