Ozawa M, Ohtsuka T, Okamura N, Ishibashi S
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Arch Biochem Biophys. 1989 Sep;273(2):491-6. doi: 10.1016/0003-9861(89)90508-0.
Treatment of guinea pig polymorphonuclear leukocytes (PMNL) with various fatty acids elicited superoxide anion (O2-) production and an increase in intracellular Ca2+ [( Ca2+]i). Both responses, however, were seldom observed when PMNL were treated at lower concentrations. But, simultaneous addition of 1-oleoyl-2-acetylglycerol (OAG), a protein kinase C activator, caused an increase in O2- production even at the lower concentrations of fatty acids. In contrast to the synergism in O2- production, [Ca2+]i remained at almost the basal level irrespective of the presence of OAG. Among saturated fatty acids, those with carbon numbers of 14 to 18 were most effective in stimulating O2- production in combination with OAG. Unsaturated fatty acids with a carbon number of 18 were almost equally effective irrespective of the number of double bonds.
用各种脂肪酸处理豚鼠多形核白细胞（PMNL）会引发超氧阴离子（O2-）的产生以及细胞内Ca2+（[Ca2+]i）浓度的升高。然而，当以较低浓度处理PMNL时，很少观察到这两种反应。但是，同时添加蛋白激酶C激活剂1-油酰基-2-乙酰甘油（OAG），即使在较低脂肪酸浓度下也会导致O2-产生增加。与O2-产生的协同作用相反，无论是否存在OAG，[Ca2+]i几乎保持在基础水平。在饱和脂肪酸中，碳数为14至18的脂肪酸与OAG联合使用时，对刺激O2-产生最有效。碳数为18的不饱和脂肪酸，无论双键数量如何，几乎同样有效。
