Ohtsuka T, Hiura M, Ozawa M, Okamura N, Ishibashi S
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Arch Biochem Biophys. 1990 May 15;279(1):21-4. doi: 10.1016/0003-9861(90)90457-a.
The superoxide anion (O2-) production of guinea pig polymorphonuclear leukocytes (PMNL) by platelet-activating factor (PAF) was greatly enhanced by the addition of 1-oleoyl-2-acetylglycerol (OAG), even at low concentrations of OAG at which O2- production was little induced. The enhanced production was biphasic, depending on concentrations of PAF. One was saturable at a lower concentration range of PAF and probably mediated through a putative PAF receptor-islet-activating protein-sensitive GTP-binding protein (G protein) pathway. Another was increased in a concentration-dependent manner at a higher concentration range of PAF and apparently mediated through both receptor-G protein-dependent and -independent pathways. These results suggest that at least two types of action of PAF are involved in the synergistic stimulation of O2- production by PAF and OAG in PMNL.
即使在低浓度的1-油酰基-2-乙酰甘油（OAG）下，豚鼠多形核白细胞（PMNL）通过血小板活化因子（PAF）产生超氧阴离子（O2-）的能力也会因添加OAG而大大增强，此时OAG对O2-产生的诱导作用很小。这种增强的产生呈双相性，取决于PAF的浓度。一种在PAF的较低浓度范围内是可饱和的，可能是通过一种假定的PAF受体-胰岛激活蛋白敏感的GTP结合蛋白（G蛋白）途径介导的。另一种在PAF的较高浓度范围内以浓度依赖性方式增加，显然是通过受体-G蛋白依赖性和非依赖性途径介导的。这些结果表明，PAF至少有两种作用类型参与了PAF和OAG对PMNL中O2-产生的协同刺激。
