Tombuloglu Guzin, Tombuloglu Huseyin, Sakcali M Serdal, Unver Turgay
Department of Biology, Faculty of Science and Arts, Fatih University, Istanbul, Turkey; Vocational High School of Health Services, Fatih University, Istanbul, Turkey.
Department of Biology, Faculty of Science and Arts, Fatih University, Istanbul, Turkey.
Gene. 2015 Feb 15;557(1):71-81. doi: 10.1016/j.gene.2014.12.012. Epub 2014 Dec 9.
Boron (B) is an essential micronutrient for optimum plant growth. However, above certain threshold B is toxic and causes yield loss in agricultural lands. While a number of studies were conducted to understand B tolerance mechanism, a transcriptome-wide approach for B tolerant barley is performed here for the first time. A high-throughput RNA-Seq (cDNA) sequencing technology (Illumina) was used with barley (Hordeum vulgare), yielding 208 million clean reads. In total, 256,874 unigenes were generated and assigned to known peptide databases: Gene Ontology (GO) (99,043), Swiss-Prot (38,266), Clusters of Orthologous Groups (COG) (26,250), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) (36,860), as determined by BLASTx search. According to the digital gene expression (DGE) analyses, 16% and 17% of the transcripts were found to be differentially regulated in root and leaf tissues, respectively. Most of them were involved in cell wall, stress response, membrane, protein kinase and transporter mechanisms. Some of the genes detected as highly expressed in root tissue are phospholipases, predicted divalent heavy-metal cation transporters, formin-like proteins and calmodulin/Ca(2+)-binding proteins. In addition, chitin-binding lectin precursor, ubiquitin carboxyl-terminal hydrolase, and serine/threonine-protein kinase AFC2 genes were indicated to be highly regulated in leaf tissue upon excess B treatment. Some pathways, such as the Ca(2+)-calmodulin system, are activated in response to B toxicity. The differential regulation of 10 transcripts was confirmed by qRT-PCR, revealing the tissue-specific responses against B toxicity and their putative function in B-tolerance mechanisms.
硼（B）是植物最佳生长所必需的微量营养素。然而，超过一定阈值的硼具有毒性，会导致农田产量损失。虽然已经进行了多项研究来了解硼耐受机制，但本文首次对耐硼大麦进行了全转录组分析。采用高通量RNA测序（cDNA）技术（Illumina）对大麦（Hordeum vulgare）进行测序，获得了2.08亿条干净 reads。通过BLASTx搜索，共生成了256,874个单基因，并将其分配到已知的肽数据库中：基因本体论（GO）（99,043个）、瑞士蛋白质数据库（Swiss-Prot）（38,266个）、直系同源簇（COG）（26,250个）和京都基因与基因组百科全书（KEGG）（36,860个）。根据数字基因表达（DGE）分析，分别在根和叶组织中发现16%和17%的转录本存在差异调控。其中大多数参与细胞壁、应激反应、膜、蛋白激酶和转运机制。在根组织中检测到的一些高表达基因是磷脂酶、预测的二价重金属阳离子转运蛋白、formin样蛋白和钙调蛋白/Ca(2+)结合蛋白。此外，几丁质结合凝集素前体、泛素羧基末端水解酶和丝氨酸/苏氨酸蛋白激酶AFC2基因在过量硼处理后的叶组织中被高度调控。一些途径，如Ca(2+)-钙调蛋白系统，会因硼毒性而被激活。通过qRT-PCR证实了10个转录本的差异调控，揭示了对硼毒性的组织特异性反应及其在硼耐受机制中的假定功能。
