Boron (B) is an essential micronutrient critical for crop growth and productivity. However, excessive boron concentrations can impair plant development, and detoxification remains a significant challenge. Understanding genetic variability and identifying tolerance mechanisms are crucial for developing boron-resistant cultivars. This study explores the physiological and molecular responses of two species, namely kiwifruit () and kiwiberry (), to varying levels of excess B. Under excessive B conditions, B accumulation followed the order roots< stems< leaves, with maximum concentrations of 68.6 mg/kg, 105 mg/kg, and 160.7 mg/kg in , and 68.2 mg/kg, 107 mg/kg, and 196.9 mg/kg in , respectively. B toxicity symptoms appeared in when B levels exceeded 50 mg/kg, leading to a 15-20% reduction in dry weight across roots, stems, and leaves. exhibited greater sensitivity, with a 20-30% reduction in dry biomass. Both species showed significant declines in chlorophyll a and b content under B stress, with alterations in the chlorophyll a/b ratio and increased oxidative stress. Additionally, stress-responsive genes, including () and (), were downregulated in response to B stress, suggesting potential disruptions in growth and development. These findings provide valuable insights into the differential physiological and molecular responses to excess boron in species, laying a foundation for functional genomics research and the development of boron-tolerant kiwifruit cultivars.