Neuhybel P, Holman J
Arch Exp Veterinarmed. 1989 May;43(3):335-9.
30 one-day-old chickens of the commercial HX-SL hybrid from the incubator house at Premyslovice were infected with tissue culture supernatant of clone LSCC-H32. This tissue culture produced the virus of avian lymphoid leucosis of subgroups A and B. After killing at the age of 21-37 weeks, samples of chick pancreas were examined histopathologically and by electron microscopy. Serological examination of serum and feather pulp for presence of avian sarcoma-leucosis gs antigen was simultaneously performed, using the ELISA test. In the pancreas of the experimental chickens both complete and incomplete viral particles of avian leucosis were found. Most frequently, they were localised in the electron-dense secretions of the lumen of pancreatic acini at apical plasma membranes of acinar cells but also in zymogenic granules and in intercellular spaces. The significance of these findings to possible horizontal transmission is discussed.
从普热米斯洛维采孵化场选取30只1日龄商品HX - SL杂交鸡，用克隆LSCC - H32的组织培养上清液进行感染。该组织培养物产生A和B亚群的禽淋巴细胞性白血病病毒。在21至37周龄时宰杀后，对鸡胰腺样本进行组织病理学检查和电子显微镜检查。同时使用酶联免疫吸附试验（ELISA）对血清和羽毛髓进行禽肉瘤 - 白血病gs抗原存在情况的血清学检测。在实验鸡的胰腺中发现了禽白血病的完整和不完整病毒颗粒。它们最常位于胰腺腺泡细胞顶端质膜处腺泡腔的电子致密分泌物中，但也存在于酶原颗粒和细胞间隙中。讨论了这些发现对可能的水平传播的意义。
