College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, China.
College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China.
Colloids Surf B Biointerfaces. 2015 Jan 1;125:213-21. doi: 10.1016/j.colsurfb.2014.11.040. Epub 2014 Dec 3.
Surface chemical characteristics of biomedical polymers, which are determined by the migration and rearrangement of polymeric chains, play an important role in the protein adsorption. In this work, the relationship between the architectures of amphiphilic polyesters and their protein adsorption resistance was investigated. Three poly (ɛ-caprolactone)s containing sulfobetaines (PCL-b-PDEAS) segments with linear, four arms and six arms star-shaped architectures were synthesized with the combination of ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP). The structures of the amphiphiles were confirmed by (1)H NMR and FTIR. Water contact angles (WCA) and X-ray photoelectron spectroscopy (XPS) were used to study the surface properties of the amphiphilic copolymer films. The water contact angles were decreased due to the surface migration of hydrophilic segments. Transmission electron microscopy (TEM) displayed the occurrence of microphase separation phenomena for PCL-b-PDEAS above glass transition temperature (Tg). The results showed that the hydrophilic segments in the copolymers would migrate to the surface of the films, which resulted in the surface more hydrophilic to resist protein adsorption. The adsorption of both fibrinogen (Fg) and bovine serum albumin (BSA) were studied. The results showed that protein adsorption was depended on not only the hydrophilic chain migration but also the shape of proteins.
生物医学聚合物的表面化学特性取决于聚合物链的迁移和重排，在蛋白质吸附中起着重要作用。在这项工作中，研究了两亲聚酯的结构与其抗蛋白质吸附能力之间的关系。通过开环聚合（ROP）和原子转移自由基聚合（ATRP）的组合，合成了三种含有磺酸甜菜碱（PCL-b-PDEAS）段的线性、四臂和六臂星形结构的聚（ε-己内酯）（PCL）。通过（1）H NMR 和 FTIR 证实了两亲物的结构。通过水接触角（WCA）和 X 射线光电子能谱（XPS）研究了两亲性共聚物薄膜的表面性质。由于亲水性链段的表面迁移，水接触角降低。透射电子显微镜（TEM）显示，在玻璃化转变温度（Tg）以上，PCL-b-PDEAS 发生了微相分离现象。结果表明，共聚物中的亲水性链段会迁移到薄膜表面，使表面更加亲水，从而抵抗蛋白质吸附。研究了纤维蛋白原（Fg）和牛血清白蛋白（BSA）的吸附。结果表明，蛋白质吸附不仅取决于亲水链的迁移，还取决于蛋白质的形状。
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