College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
Colloids Surf B Biointerfaces. 2012 Dec 1;100:116-25. doi: 10.1016/j.colsurfb.2012.05.004. Epub 2012 May 24.
In this study, a novel triblock copolymer of poly (styrene-co-acrylic acid)-b-poly (vinyl pyrrolidone)-b-poly(styrene-co-acrylic acid) (P(St-co-AA)-b-PVP-b-P(St-co-AA)) is synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization, and used for the modification of blood contacting surface of polyethersulfone (PES) membrane to improve blood compatibility. The synthesized block copolymer can be directly blended with PES to prepare PES membranes by a liquid-liquid phase separation technique. The compositions and structure of the PES membranes are characterized by thermogravimetric analysis (TGA), ATR-FTIR, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM); the surface charge density of the modified PES membrane was measured by Zeta-potential; the blood compatibility of the PES membranes was assessed by detecting bovine serum albumin (BSA) and bovine serum fibrinogen (BFG) adsorption, platelet adhesion, activated partial thromboplastin time (APTT), platelet activation, and thrombin-antithrombin III (TAT) generation. The results indicated that the blood compatibility of the modified PES membrane was improved due to the membrane surface modification by blending the amphiphilic block copolymer and the surface segregation of the block copolymer.
在这项研究中，通过可逆加成-断裂链转移（RAFT）聚合合成了一种新型的嵌段共聚物，聚（苯乙烯-co-丙烯酸）-b-聚（聚乙烯基吡咯烷酮）-b-聚（苯乙烯-co-丙烯酸）（P(St-co-AA)-b-PVP-b-P(St-co-AA)），并将其用于修饰聚醚砜（PES）膜的血液接触表面，以提高血液相容性。合成的嵌段共聚物可以直接与 PES 共混，通过液-液相分离技术制备 PES 膜。PES 膜的组成和结构通过热重分析（TGA）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）、X 射线光电子能谱（XPS）和扫描电子显微镜（SEM）进行表征；通过 Zeta 电位测量改性 PES 膜的表面电荷密度；通过检测牛血清白蛋白（BSA）和牛血清纤维蛋白原（BFG）吸附、血小板黏附、活化部分凝血活酶时间（APTT）、血小板活化和凝血酶-抗凝血酶 III（TAT）生成来评估 PES 膜的血液相容性。结果表明，由于共混亲水性嵌段共聚物对膜表面进行了修饰以及嵌段共聚物的表面分相，改性 PES 膜的血液相容性得到了提高。
