Sharma Manish, Singh Devinder, Sharma Ajay Kumar
Department of Zoology & Environmental Sciences, Punjabi University, Patiala 147002, India.
Department of Zoology & Environmental Sciences, Punjabi University, Patiala 147002, India.
Forensic Sci Int. 2015 Feb;247:1-6. doi: 10.1016/j.forsciint.2014.11.017. Epub 2014 Nov 24.
An absolutely vital prerequisite in the forensic entomology cases is the estimation of post mortem interval (PMI). Due to similar morphological look, identification of the flesh fly fauna associated with the corpse is very difficult for nontaxonomists and needs professional hand to be dealt with. So, to simplify the identification process the application of 465 bp fragment of COI gene for differentiation of ten forensically significant species of flesh flies is demonstrated in this paper. Percentage nucleotide composition, genetic divergence and substitution rate were calculated by using the Maximum likelihood method. Phylogenetic analysis was done by Neighbour-joining tree constructed by using Tamura-3-parameter given in the MEGA5 software. The results show the robustness of COI gene as diagnostic marker, since its nucleotide variability enables dependable distinction to be drawn between species.
在法医昆虫学案例中，一个绝对至关重要的前提是死后间隔时间（PMI）的估计。由于形态外观相似，对于非分类学家来说，识别与尸体相关的麻蝇类群非常困难，需要专业人员来处理。因此，为了简化识别过程，本文展示了应用细胞色素氧化酶亚基I（COI）基因的465 bp片段来区分十种具有法医意义的麻蝇物种。通过最大似然法计算核苷酸组成百分比、遗传距离和替换率。使用MEGA5软件中给出的 Tamura-3-参数构建邻接树进行系统发育分析。结果表明COI基因作为诊断标记具有稳健性，因为其核苷酸变异性能够可靠地区分不同物种。
