Meiklejohn Kelly A, Wallman James F, Dowton Mark
Institute for Conservation Biology and Environmental Management, School of Biological Sciences, University of Wollongong, NSW, 2522, Australia.
J Forensic Sci. 2013 Jan;58(1):184-7. doi: 10.1111/j.1556-4029.2012.02220.x.
Carrion-breeding insects, such as flesh flies (Diptera: Sarcophagidae), can be used as evidence in forensic investigations. Despite their considerable forensic potential, their use has been limited because morphological species identification, at any life stage, is very challenging. This study investigated whether DNA could be extracted and cytochrome oxidase subunit I (COI) barcode sequences obtained for molecular identification of each immature life stage of the forensically important Australian flesh fly, Sarcophaga (Sarcorohdendorfia) impatiens (Walker). Genomic DNA extracts were prepared from all larval instars and puparia. Amplification of the barcoding region was successful from all extracts, but puparia amplicons were weak. All sequences were identified as S. impatiens with 99.95% confidence using the Barcoding of Life Database (BOLD). Importantly, crop removal was necessary to eliminate PCR inhibition for specimens from late second and early third instars. Similar results are expected for immatures of other carrion-breeding species, enhancing the use of evidence from immature flies in forensic investigations.
以腐肉为食的昆虫，如麻蝇（双翅目：麻蝇科），可作为法医调查的证据。尽管它们具有相当大的法医应用潜力，但由于在任何生命阶段进行形态学物种鉴定都极具挑战性，其应用一直受到限制。本研究调查了是否可以提取DNA并获得细胞色素氧化酶亚基I（COI）条形码序列，用于对具有法医重要性的澳大利亚麻蝇（Sarcophaga (Sarcorohdendorfia) impatiens (Walker)）的每个未成熟生命阶段进行分子鉴定。从所有幼虫龄期和蛹制备了基因组DNA提取物。所有提取物均成功扩增出条形码区域，但蛹的扩增产物较弱。使用生命条形码数据库（BOLD）以99.95%的置信度将所有序列鉴定为S. impatiens。重要的是，对于来自第二龄后期和第三龄初期的标本，去除嗉囊对于消除PCR抑制是必要的。预计其他以腐肉为食的物种的未成熟个体也会有类似结果，这将增加未成熟苍蝇在法医调查中的证据利用。
