Xiang Y, Huang S F, Xia J R, Ye D Q, Chen P, Yang S S, Sun S, Lai X F, Zhang L P
Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, China
Genet Mol Res. 2014 Nov 7;13(4):9187-95. doi: 10.4238/2014.November.7.5.
An association between the sequence variants of cytokine genes and various clinical outcomes in subjects infected with the hepatitis B virus (HBV) has been demonstrated. However, the results are inconsistent and inconclusive. Further studies in other populations and the evaluation of a greater number of individuals may contribute to a better understanding of the influence of the cytokine genetic variants on the evolution of HBV infections. This study was performed to explore the relationships between the sequence variants of TNF-A-308, IFNAR1-17470, and IL-10-592 and the susceptibility to chronic hepatitis B (CHB) in a Chinese population. A total of 160 patients with CHB and 124 individuals who had spontaneously recovered (SR) from hepatitis B were enrolled in the present study. The variants at TNF-A-308, IFNAR1-17470, and IL-10-592 were determined by PCR-restriction fragment length polymorphism analysis and were confirmed by bidirectional DNA sequencing. Significant differences were found between the CHB and the SR groups in the frequency and distribution of the genotypes of both IFNAR1-17470 and IL-10-592 genes. In comparison with the CHB patients with the IFNAR1-17470 G/G variant, the odds ratio (OR) of the CHB patients with the IFNAR1-17470 C/C variant developing chronic hepatitis was 2.06 (95%CI = 1.03-4.14). In addition, the OR of the patients with CHB having the IL-10-592 C/C variant developing chronic hepatitis was 2.77 (95%CI = 1.13-4.57) when compared with that of the patients with the IL-10-592 A/A variant. In conclusion, sequence variants of both the IFNAR1-17470 and IL-10-592 genes were correlated with susceptibility to CHB.
细胞因子基因序列变异与乙型肝炎病毒（HBV）感染患者的各种临床结局之间的关联已得到证实。然而，结果并不一致且尚无定论。在其他人群中开展进一步研究以及评估更多个体可能有助于更好地理解细胞因子基因变异对HBV感染演变的影响。本研究旨在探讨肿瘤坏死因子 - A（TNF - A）-308、Ⅰ型干扰素受体1（IFNAR1）-17470和白细胞介素10（IL - 10）-592的序列变异与中国人群慢性乙型肝炎（CHB）易感性之间的关系。本研究共纳入160例CHB患者和124例已从乙型肝炎中自发康复（SR）的个体。通过聚合酶链反应 - 限制性片段长度多态性分析确定TNF - A - 308、IFNAR1 - 17470和IL - 10 - 592的变异，并通过双向DNA测序进行确认。在IFNAR1 - 17470和IL - 10 - 592基因的基因型频率和分布方面，CHB组与SR组之间存在显著差异。与具有IFNAR1 - 17470 G/G变异的CHB患者相比，具有IFNAR1 - 17470 C/C变异的CHB患者发生慢性肝炎的比值比（OR）为2.06（95%置信区间[CI]=1.03 - 4.14）。此外，与具有IL - 10 - 592 A/A变异的患者相比，具有IL - 10 - 592 C/C变异的CHB患者发生慢性肝炎的OR为2.77（95%CI = 1.13 - 4.57）。总之，IFNAR1 - 17470和IL - 10 - 592基因的序列变异均与CHB易感性相关。
