Costa M C, Camargo C, Laganaro N M, Oliveira P R R, Davanço P V, Azeredo R M A, Simpson J G P, Silveira L F, Francisco M R
Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.
CRAX, Sociedade de Pesquisa do Manejo e da Reprodução da Fauna Silvestre, Contagem, MG, Brasil.
Genet Mol Res. 2014 Nov 27;13(4):9867-73. doi: 10.4238/2014.November.27.14.
Cracids are medium to large frugivorous birds that are endemic to the Neotropics. Because of deforestation and overhunting, many species are threatened. The conservation of several species has relied on captive breeding and reintroduction in the wild, but captive populations may be inbred. Microsatellite tools can permit the construction of genetic pedigrees to reduce inbreeding, but only a few loci are available for this group of birds. Here, we present 10 novel polymorphic microsatellite loci and the cross-amplification of these and of 10 additional loci available in the literature in a panel of 5 cracid species, including 3 species with high conservation concern. We provide the first polymorphic loci for the jacutinga, Aburria jacutinga (N = 8), and red-billed curassow, Crax blumenbachii (N = 9), and additional loci for bare-faced curassow, C. fasciolata (N = 8), Alagoas curassow, Pauxi mitu (N = 5), and razor-billed curassow, P. tuberosa (N = 5). The average number of alleles was 2.9 for A. jacutinga, 2.7 for C. blumenbachii, 3.5 for C. fasciolata, 2.6 for P. mitu, and 5.7 for P. tuberosa. The mean expected heterozygosities were 0.42, 0.40, 0.48, 0.37, and 0.59, respectively. The average probabilities that the set of loci would not exclude a pair of parents of an arbitrary offspring were 2.9% in A. jacutinga, 1% in C. blumenbachii, 0.5% in C. fasciolata, 0.4% in P. mitu, and 0.002% in P. tuberosa suggesting that these loci may be adequate for parentage analysis and to implement ex situ genetic management plans.
凤冠雉科鸟类是中型至大型的食果性鸟类，为新热带界特有。由于森林砍伐和过度捕猎，许多物种受到威胁。一些物种的保护依赖于圈养繁殖和野外放归，但圈养种群可能存在近亲繁殖现象。微卫星工具可用于构建遗传谱系以减少近亲繁殖，但针对这类鸟类的可用位点较少。在此，我们展示了10个新的多态性微卫星位点，以及这些位点和文献中另外10个位点在5种凤冠雉科鸟类中的交叉扩增情况，这5种鸟类包括3种具有高度保护关切的物种。我们为尖羽冠雉（Aburria jacutinga，样本量N = 8）和红嘴凤冠雉（Crax blumenbachii，样本量N = 9）提供了首个多态性位点，为裸面凤冠雉（C. fasciolata，样本量N = 8）、阿拉戈斯凤冠雉（Pauxi mitu，样本量N = 5）和刀嘴凤冠雉（P. tuberosa，样本量N = 5）提供了额外的位点。尖羽冠雉的平均等位基因数为2.9个，红嘴凤冠雉为2.7个，裸面凤冠雉为3.5个，阿拉戈斯凤冠雉为2.6个，刀嘴凤冠雉为5.7个。平均期望杂合度分别为0.42、0.40、0.48、0.37和0.59。这些位点不排除任意后代一对亲本的平均概率在尖羽冠雉中为2.9%，在红嘴凤冠雉中为1%，在裸面凤冠雉中为0.5%，在阿拉戈斯凤冠雉中为0.4%，在刀嘴凤冠雉中为0.002%，这表明这些位点可能适用于亲权分析和实施迁地遗传管理计划。
