大鼠腹内侧下丘脑的FTO基因敲低不影响能量平衡。

van Gestel Margriet A, Sanders Loek E, de Jong Johannes W, Luijendijk Mieneke C M, Adan Roger A H

Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht, Utrecht, The Netherlands.

Physiol Rep. 2014 Dec 11;2(12). doi: 10.14814/phy2.12152. Print 2014 Dec 1.

Single nucleotide polymorphisms (SNPs) clustered in the first intron of the fat mass and obesity-associated (FTO) gene has been associated with obesity. FTO expression is ubiquitous, with particularly high levels in the hypothalamic area of the brain. To investigate the region-specific role of FTO, AAV technology was applied to knockdown FTO in the ventromedial hypothalamus (VMH). No effect of FTO knockdown was observed on bodyweight or parameters of energy balance. Animals were exposed twice to an overnight fast, followed by a high-fat high-sucrose (HFHS) diet for 1 week. FTO knockdown did not result in a different response to the diets. A region-specific role for FTO in the VMH in the regulation of energy balance could not be found.

聚集在脂肪量和肥胖相关（FTO）基因第一个内含子中的单核苷酸多态性（SNP）与肥胖有关。FTO表达广泛存在，在大脑下丘脑区域水平尤其高。为了研究FTO的区域特异性作用，采用腺相关病毒（AAV）技术在下丘脑腹内侧核（VMH）中敲低FTO。未观察到FTO敲低对体重或能量平衡参数有影响。动物两次经历过夜禁食，随后给予高脂高糖（HFHS）饮食1周。FTO敲低并未导致对饮食有不同反应。未发现FTO在VMH中对能量平衡调节有区域特异性作用。