Campbell Michael S, Holt Carson, Moore Barry, Yandell Mark
Eccles Institute of Human Genetics, University of Utah, Salt Lake City, Utah.
USTAR Center for Genetic Discovery, University of Utah, Salt Lake City, Utah.
Curr Protoc Bioinformatics. 2014 Dec 12;48:4.11.1-4.11.39. doi: 10.1002/0471250953.bi0411s48.
This unit describes how to use the genome annotation and curation tools MAKER and MAKER-P to annotate protein-coding and noncoding RNA genes in newly assembled genomes, update/combine legacy annotations in light of new evidence, add quality metrics to annotations from other pipelines, and map existing annotations to a new assembly. MAKER and MAKER-P can rapidly annotate genomes of any size, and scale to match available computational resources.
本单元介绍了如何使用基因组注释和管理工具MAKER和MAKER-P，对新组装的基因组中的蛋白质编码基因和非编码RNA基因进行注释，根据新证据更新/合并旧注释，为其他流程的注释添加质量指标，以及将现有注释映射到新组装的基因组上。MAKER和MAKER-P可以快速注释任何大小的基因组，并能根据可用的计算资源进行扩展。
