膜蛋白成功结晶的方法。

Methods for the successful crystallization of membrane proteins.

作者信息

Moraes Isabel, Archer Margarida

机构信息

Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK,

出版信息

Methods Mol Biol. 2015;1261:211-30. doi: 10.1007/978-1-4939-2230-7_12.

DOI:10.1007/978-1-4939-2230-7_12

PMID:25502202

Abstract

In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with structure determination of membrane proteins by X-ray crystallography. The advent of genomics and proteomics initiatives combined with high-throughput technologies, such as automation, miniaturization, integration, and third-generation synchrotrons, has enhanced membrane protein structure determination rate. Nevertheless, crystallization of membrane proteins still remains one of the most troublesome hurdles that every structural group must undertake. This chapter presents high-throughput methods easily available to any researcher interested in membrane protein characterization and crystallization. It is our hope this chapter can be used as a positive guide to all who are attempting crystallizing membrane proteins.

摘要

近年来，人们付出了诸多努力进行创新开发，以克服与通过X射线晶体学测定膜蛋白结构相关的众多障碍。基因组学和蛋白质组学计划的出现，与自动化、小型化、集成化以及第三代同步加速器等高通量技术相结合，提高了膜蛋白结构的测定率。然而，膜蛋白的结晶仍然是每个结构研究团队都必须面对的最棘手的障碍之一。本章介绍了任何对膜蛋白表征和结晶感兴趣的研究人员都易于采用的高通量方法。我们希望本章能为所有试图结晶膜蛋白的人提供积极的指导。