Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Structure. 2011 Oct 12;19(10):1381-93. doi: 10.1016/j.str.2011.09.001.
Electron crystallography is a powerful technique for the study of membrane protein structure and function in the lipid environment. When well-ordered two-dimensional crystals are obtained the structure of both protein and lipid can be determined and lipid-protein interactions analyzed. Protons and ionic charges can be visualized by electron crystallography and the protein of interest can be captured for structural analysis in a variety of physiologically distinct states. This review highlights the strengths of electron crystallography and the momentum that is building up in automation and the development of high throughput tools and methods for structural and functional analysis of membrane proteins by electron crystallography.
电子晶体学是研究膜蛋白在脂质环境中的结构和功能的有力技术。当获得有序的二维晶体时，可以确定蛋白质和脂质的结构，并分析脂质-蛋白质相互作用。通过电子晶体学可以可视化质子和离子电荷，并且可以捕获感兴趣的蛋白质以在多种生理上不同的状态下进行结构分析。这篇综述强调了电子晶体学的优势，以及自动化的发展势头，以及开发高通量工具和方法用于电子晶体学研究膜蛋白的结构和功能。
