电子晶体学在膜蛋白结构与功能分析方面的进展。
Advances in structural and functional analysis of membrane proteins by electron crystallography.
机构信息
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
出版信息
Structure. 2011 Oct 12;19(10):1381-93. doi: 10.1016/j.str.2011.09.001.
Abstract
Electron crystallography is a powerful technique for the study of membrane protein structure and function in the lipid environment. When well-ordered two-dimensional crystals are obtained the structure of both protein and lipid can be determined and lipid-protein interactions analyzed. Protons and ionic charges can be visualized by electron crystallography and the protein of interest can be captured for structural analysis in a variety of physiologically distinct states. This review highlights the strengths of electron crystallography and the momentum that is building up in automation and the development of high throughput tools and methods for structural and functional analysis of membrane proteins by electron crystallography.
摘要
电子晶体学是研究膜蛋白在脂质环境中的结构和功能的有力技术。当获得有序的二维晶体时，可以确定蛋白质和脂质的结构，并分析脂质-蛋白质相互作用。通过电子晶体学可以可视化质子和离子电荷，并且可以捕获感兴趣的蛋白质以在多种生理上不同的状态下进行结构分析。这篇综述强调了电子晶体学的优势，以及自动化的发展势头，以及开发高通量工具和方法用于电子晶体学研究膜蛋白的结构和功能。
相似文献
1
Advances in structural and functional analysis of membrane proteins by electron crystallography.电子晶体学在膜蛋白结构与功能分析方面的进展。
Structure. 2011 Oct 12;19(10):1381-93. doi: 10.1016/j.str.2011.09.001.
2
Electron crystallography of membrane proteins: two-dimensional crystallization and screening by electron microscopy.膜蛋白的电子晶体学：二维结晶及电子显微镜筛选
Methods. 2007 Apr;41(4):417-26. doi: 10.1016/j.ymeth.2006.07.011.
3
Introduction to electron crystallography.电子晶体学导论。
Methods Mol Biol. 2013;955:1-16. doi: 10.1007/978-1-62703-176-9_1.
4
Use of detergents in two-dimensional crystallization of membrane proteins.洗涤剂在膜蛋白二维结晶中的应用。
Biochim Biophys Acta. 2000 Nov 23;1508(1-2):112-28. doi: 10.1016/s0005-2736(00)00307-2.
5
Electron crystallography for structural and functional studies of membrane proteins.用于膜蛋白结构与功能研究的电子晶体学
J Electron Microsc (Tokyo). 2011;60 Suppl 1:S149-59. doi: 10.1093/jmicro/dfr033.
6
High-throughput methods for electron crystallography.电子晶体学的高通量方法。
Methods Mol Biol. 2013;955:273-96. doi: 10.1007/978-1-62703-176-9_15.
7
Electron crystallography as a technique to study the structure on membrane proteins in a lipidic environment.电子晶体学作为一种在脂质环境中研究膜蛋白结构的技术。
Annu Rev Biophys. 2009;38:89-105. doi: 10.1146/annurev.biophys.050708.133649.
8
Asymmetric binding of membrane proteins to GroEL.膜蛋白与GroEL的不对称结合。
Arch Biochem Biophys. 2005 Feb 15;434(2):352-7. doi: 10.1016/j.abb.2004.11.021.
9
Inducing two-dimensional crystallization of membrane proteins by dialysis for electron crystallography.通过透析诱导膜蛋白二维结晶用于电子晶体学研究
Methods Enzymol. 2015;557:351-62. doi: 10.1016/bs.mie.2014.12.022. Epub 2015 Mar 24.
10
New approaches towards the understanding of integral membrane proteins: A structural perspective on G protein-coupled receptors.理解整合膜蛋白的新方法：G蛋白偶联受体的结构视角
Protein Sci. 2017 Aug;26(8):1493-1504. doi: 10.1002/pro.3200. Epub 2017 Jun 7.
引用本文的文献
1
An Overview of Microcrystal Electron Diffraction (MicroED).微晶体电子衍射（MicroED）概述。
Annu Rev Biochem. 2021 Jun 20;90:431-450. doi: 10.1146/annurev-biochem-081720-020121.
2
Beyond protein structure determination with MicroED.超越蛋白结构测定的 MicroED 技术。
Curr Opin Struct Biol. 2020 Oct;64:51-58. doi: 10.1016/j.sbi.2020.05.018. Epub 2020 Jun 28.
3
In-Line Holography in Transmission Electron Microscopy for the Atomic Resolution Imaging of Single Particle of Radiation-Sensitive Matter.透射电子显微镜中的在线全息术用于对辐射敏感物质单颗粒进行原子分辨率成像
Materials (Basel). 2020 Mar 20;13(6):1413. doi: 10.3390/ma13061413.
4
The cryo-EM method microcrystal electron diffraction (MicroED).低温电子显微镜方法——微晶体电子衍射（MicroED）。
Nat Methods. 2019 May;16(5):369-379. doi: 10.1038/s41592-019-0395-x. Epub 2019 Apr 29.
5
Emerging Diversity in Lipid-Protein Interactions.脂质-蛋白质相互作用的新多样性。
Chem Rev. 2019 May 8;119(9):5775-5848. doi: 10.1021/acs.chemrev.8b00451. Epub 2019 Feb 13.
6
Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel.工程化交联用于研究CRAC通道的孔结构
Methods Mol Biol. 2018;1843:147-166. doi: 10.1007/978-1-4939-8704-7_13.
7
Two-Dimensional Crystallization Procedure, from Protein Expression to Sample Preparation.二维结晶程序：从蛋白质表达至样品制备
Biomed Res Int. 2015;2015:693869. doi: 10.1155/2015/693869. Epub 2015 Aug 27.
8
Protein structure determination by MicroED.通过微晶电子衍射进行蛋白质结构测定。
Curr Opin Struct Biol. 2014 Aug;27:24-31. doi: 10.1016/j.sbi.2014.03.004. Epub 2014 Apr 5.
9
Gently does it for submicron crystals.对于亚微米晶体要轻柔操作。
Elife. 2013 Nov 19;2:e01662. doi: 10.7554/eLife.01662.
10
Three-dimensional electron crystallography of protein microcrystals.蛋白质微晶的三维电子晶体学
Elife. 2013 Nov 19;2:e01345. doi: 10.7554/eLife.01345.
本文引用的文献
1
Fragment-based phase extension for three-dimensional structure determination of membrane proteins by electron crystallography.基于片段的相位扩展在电子晶体学中用于确定膜蛋白的三维结构。
Structure. 2011 Jul 13;19(7):976-87. doi: 10.1016/j.str.2011.04.008.
2
Improved molecular replacement by density- and energy-guided protein structure optimization.密度和能量引导的蛋白质结构优化提高分子置换
Nature. 2011 May 26;473(7348):540-3. doi: 10.1038/nature09964. Epub 2011 May 1.
3
Conformational rearrangement of gastric H(+),K(+)-ATPase induced by an acid suppressant.一种抑酸剂诱导的胃H⁺，K⁺-ATP酶的构象重排
Nat Commun. 2011 Jan 11;2:155. doi: 10.1038/ncomms1154.
4
Images of paraffin monolayer crystals with perfect contrast: minimization of beam-induced specimen motion.具有完美对比度的石蜡单层晶体图像：最小化束诱导的样品运动。
Ultramicroscopy. 2011 Jan;111(2):90-100. doi: 10.1016/j.ultramic.2010.10.010. Epub 2010 Oct 26.
5
Asymmetric configurations and N-terminal rearrangements in connexin26 gap junction channels.连接蛋白 26 缝隙连接通道中的不对称构象和 N 端重排。
J Mol Biol. 2011 Jan 21;405(3):724-35. doi: 10.1016/j.jmb.2010.10.032. Epub 2010 Nov 20.
6
Automated screening of 2D crystallization trials using transmission electron microscopy: a high-throughput tool-chain for sample preparation and microscopic analysis.利用透射电子显微镜进行二维结晶试验的自动化筛选：用于样品制备和微观分析的高通量工具链。
J Struct Biol. 2011 Feb;173(2):365-74. doi: 10.1016/j.jsb.2010.09.019. Epub 2010 Sep 22.
7
Automatic TEM image analysis of membranes for 2D crystal detection.自动 TEM 图像分析膜用于 2D 晶体检测。
Adv Exp Med Biol. 2010;680:327-33. doi: 10.1007/978-1-4419-5913-3_37.
8
Principles of membrane protein interactions with annular lipids deduced from aquaporin-0 2D crystals.从水通道蛋白 0 的 2D 晶体推断出环形脂质与膜蛋白相互作用的原理。
EMBO J. 2010 May 19;29(10):1652-8. doi: 10.1038/emboj.2010.68. Epub 2010 Apr 13.
9
An automated pipeline to screen membrane protein 2D crystallization.一种用于筛选膜蛋白二维结晶的自动化流程。
J Struct Funct Genomics. 2010 Jun;11(2):155-66. doi: 10.1007/s10969-010-9088-5. Epub 2010 Mar 27.
10
Automated electron microscopy for evaluating two-dimensional crystallization of membrane proteins.自动化电子显微镜用于评估膜蛋白的二维结晶。
J Struct Biol. 2010 Jul;171(1):102-10. doi: 10.1016/j.jsb.2010.02.018. Epub 2010 Mar 1.
Zotero 插件