基因剂量对苏云金芽孢杆菌库尔斯塔克亚种δ-内毒素基因在枯草芽孢杆菌和巨大芽孢杆菌中表达的影响。
Gene dosage effect on the expression of the delta-endotoxin genes of Bacillus thuringiensis subsp. kurstaki in Bacillus subtilis and Bacillus megaterium.
作者信息
Shivakumar A G, Vanags R I, Wilcox D R, Katz L, Vary P S, Fox J L
机构信息
Corporate Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
出版信息
Gene. 1989 Jun 30;79(1):21-31. doi: 10.1016/0378-1119(89)90089-9.
Significant differences in expression of the delta-endotoxin genes cryA1 and cryA2 of Bacillus thuringiensis subsp. kurstaki were observed in B. subtilis and B. megaterium. The cryA1 gene was expressed when present on a high-copy-number (hcn) vector in B. megaterium but not in B. subtilis. The cryA2 gene was expressed in both hosts, but at a higher level in B. megaterium. Expression of the cryA2 gene in B. megaterium was better from a hcn vector than from a low copy number vector. Inhibition of sporulation was observed when the toxin genes were present on hcn plasmids in B. subtilis while no such effect was evident in B. megaterium. In addition, there was a significant reduction in copy numbers in both B. subtilis and B. megaterium when delta-endotoxin genes or a spoVG promoter-containing fragment of DNA were cloned into hcn plasmids.
在枯草芽孢杆菌和巨大芽孢杆菌中观察到苏云金芽孢杆菌库尔斯塔克亚种的δ-内毒素基因cryA1和cryA2表达存在显著差异。cryA1基因存在于巨大芽孢杆菌的高拷贝数（hcn）载体上时可表达，但在枯草芽孢杆菌中不表达。cryA2基因在两种宿主中均有表达，但在巨大芽孢杆菌中的表达水平更高。cryA2基因在巨大芽孢杆菌中从hcn载体表达比从低拷贝数载体表达更好。当毒素基因存在于枯草芽孢杆菌的hcn质粒上时，观察到芽孢形成受到抑制，而在巨大芽孢杆菌中未观察到这种明显影响。此外，当将δ-内毒素基因或含spoVG启动子的DNA片段克隆到hcn质粒中时，枯草芽孢杆菌和巨大芽孢杆菌中的拷贝数均显著减少。
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