通过眼部接种致使人类感染性角膜炎的细菌对家兔进行组织病理学研究。
Histopathological Studies on Rabbits Infected by Bacteria Causing Infectious Keratitis in Human through Eye Inoculation.
作者信息
Aldebasi Yousef H, Mohamed Hala A, Aly Salah M
机构信息
Dept of Optometry, College of Applied Medical Sciences, Qassim University, KSA.Dept of Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Dept of Medical Laboratories, College of Applied Medical Sciences, Qassim University, KSA.Dept of Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
出版信息
Int J Health Sci (Qassim). 2014 Jul;8(3):257-67. doi: 10.12816/0023978.
AIM
This study aimed to investigate the pathogenic effect of bacteria causing infectious keratitis among patients through experimental study conducted on rabbits' eyes with the aid of histopathology as eye infection is a common disease in developing countries that may complicate to loss of vision.
METHODOLOGY
100 swab samples were collected from human infected eyes, at Qassim region during 2012, for the isolation of Pseudomonas aeruginosa and Staphylococcus aureus. The isolated pathogenic bacteria were tested to various antibiotics using some selected antibiotics discs through agar-well diffusion method. Then, experimental study conducted on 27 rabbits. The rabbits were divided randomly into three equal groups, each containing 9 rabbits. Rabbits of group (1) served as control group (Negative Control) and their eyes were inoculated with the buffer only. Rabbits of group (2) were inoculated through eyes with the isolated Pseudomonas aeruginosa. Rabbits of group (3) were inoculated through eyes with the isolated Staphylococcus aureus.
RESULTS
Out of 100 collected swab samples from human infected eyes, Pseudomonas aeruginosa and Staphylococcus aureus were isolated with a total percentage of 25.21% and 15.65%; respectively and used in this study. Both bacterial isolates were sensitive to Gentamicin and Cefuroxime. Clinically, experimentally infected rabbits by Pseudomonas aeruginosa, revealed varying degree corneal abrasions, corneal abscess and dense corneal opacity. Histopathologically, at 3(rd) day post-infection (PI), the cornea revealed polymorpho-nuclear cells infiltration with loss of the outer epithelial lining. At 7(th) day PI, neutrophils were seen in the stroma. At 15(th) day PI, proliferation of fibroblasts and new vascularisation were seen in the stroma. Clinically, rabbits experimentally infected with Staphylococcus aureus, revealed corneal ulcers and focal abscesses. Histopathologically, at 3(rd) and 7(th) day PI, the cornea revealed edema and infiltration of leukocytes. At 15(th) day PI, hyperplasia of corneal epithelium and proliferation of keratocytes were evident. The liver and kidneys of experimented rabbits revealed no remarkable histopathological alterations along the period of experiment.
CONCLUSION
Pseudomonas aeruginosa and Staphylococcus aureus are common eye infection in human, both induced severe lesions in the eyes of rabbits that could interfere with vision, therefore, strict measures to control these infections in human is recommended.
目的
本研究旨在通过对兔眼进行实验研究，并借助组织病理学方法，调查引起感染性角膜炎的细菌对患者的致病作用，因为眼部感染在发展中国家是一种常见疾病，可能会导致视力丧失等并发症。
方法
2012年在卡西姆地区从人类感染的眼睛中采集了100份拭子样本，用于分离铜绿假单胞菌和金黄色葡萄球菌。通过琼脂孔扩散法，使用一些选定的抗生素圆盘对分离出的病原菌进行多种抗生素测试。然后，对27只兔子进行实验研究。将兔子随机分为三组，每组9只。第（1）组兔子作为对照组（阴性对照），其眼睛仅接种缓冲液。第（2）组兔子通过眼睛接种分离出的铜绿假单胞菌。第（3）组兔子通过眼睛接种分离出的金黄色葡萄球菌。
结果
在从人类感染眼睛采集的100份拭子样本中，分离出铜绿假单胞菌和金黄色葡萄球菌，其总百分比分别为25.21%和15.65%，并用于本研究。两种细菌分离株对庆大霉素和头孢呋辛敏感。临床上，经铜绿假单胞菌实验感染的兔子表现出不同程度的角膜擦伤、角膜脓肿和致密的角膜混浊。组织病理学上，感染后第3天，角膜显示多形核细胞浸润，外层上皮 lining 缺失。感染后第7天，在基质中可见中性粒细胞。感染后第15天，在基质中可见成纤维细胞增殖和新血管形成。临床上，经金黄色葡萄球菌实验感染的兔子表现出角膜溃疡和局灶性脓肿。组织病理学上，感染后第3天和第7天，角膜显示水肿和白细胞浸润。感染后第15天，角膜上皮增生和角膜细胞增殖明显。实验兔子的肝脏和肾脏在实验期间未显示明显的组织病理学改变。
结论
铜绿假单胞菌和金黄色葡萄球菌是人类常见的眼部感染菌，两者均在兔眼中引起严重病变，可能影响视力，因此，建议采取严格措施控制人类中的这些感染。
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