DeCarlo J D, Van Horn D L, Hyndiuk R A, Davis S D
Arch Ophthalmol. 1981 Sep;99(9):1614-7. doi: 10.1001/archopht.1981.03930020488019.
Vitamin A-deficient rabbits were used to evaluate the role of secondary bacterial infection in the development of keratomalacia and to describe the resultant clinical and morphologic alterations. The conjunctival sacs of vitamin A-deficient rabbits at different stages of corneal involvement were inoculated with Pseudomonas aeruginosa topically. Approximately two weeks after inoculation, corneal ulceration with stromal melting developed in one of three eyes with severe punctate keratitis and in four of seven eyes with xerosis. Ulceration did not develop in any of the eight eyes with early epithelial graying or mild punctate keratitis. Inflammatory cells (primarily polymorphonuclear leukocytes) infiltrated the anterior corneal stroma of infected corneas. Liquefaction of collagen was observed in association with bacteria alone, as well as in association with polymorphonuclear leukocytes. No signs of infection were observed after conjunctival inoculation of Pseudomonas in the eyes of nine control rabbits.
使用维生素A缺乏的兔子来评估继发性细菌感染在角膜软化症发展中的作用，并描述由此产生的临床和形态学改变。将铜绿假单胞菌局部接种于处于角膜受累不同阶段的维生素A缺乏兔子的结膜囊。接种后约两周，三只患有严重点状角膜炎的眼睛中有一只以及七只患有角膜干燥症的眼睛中有四只出现了伴有基质溶解的角膜溃疡。八只出现早期上皮灰白色或轻度点状角膜炎的眼睛中均未发生溃疡。炎性细胞（主要是多形核白细胞）浸润受感染角膜的前角膜基质。观察到仅与细菌相关以及与多形核白细胞相关的胶原液化。在九只对照兔子的眼睛结膜接种铜绿假单胞菌后未观察到感染迹象。
