氨基胍与强力霉素全身给药对糖尿病大鼠改良 Widman 瓣术后牙槽骨吸收期的影响：一项组织病理学和扫描电子显微镜（SEM）研究

The Effect of Systemic Delivery of Aminoguanidine versus Doxycycline on the Resorptive Phase of Alveolar Bone Following modified Widman Flap in Diabetic Rats: A Histopathological and Scanning Electron Microscope (SEM) study.

作者信息

Tella E, Aldahlawi S, Eldeeb A, El Gazaerly H

机构信息

Professor of Periodontology, Basic and Clinical Oral Sciences Department, College of Dentistry, Umm al Qurra University, Makkah al Mokrama.

Assistance Professor of Periodontology, Basic and Clinical Oral Sciences Department, College of Dentistry, Umm al Qurra University, Makkah al Mokrama.

出版信息

Int J Health Sci (Qassim). 2014 Jul;8(3):275-85. doi: 10.12816/0023980.

DOI:10.12816/0023980

PMID:25505863

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4257363/

Abstract

OBJECTIVES

Aminoguanidine (guanylhydrazinehydrochloride) is a drug that prevents many of the classical systemic complications of diabetes including diabetic osteopenia through its inhibitory activity on the accumulation of advanced glycation end -products (AGEs). The aim of the present study was to evaluate the effectiveness of aminoguanidine versus doxycycline in reducing alveolar bone resorption following mucoperiosteal flap in diabetic rats, using the conventional histopathology and scanning electron microscope (SEM).

METHODS

Twenty-seven male albino rats were used in this study. Periodontal defects were induced experimentally on lower anterior teeth. All rats were subjected to induction of diabetes, by IV injection of the pancreatic B-cells toxin alloxan monohydrate. After eight weeks following the establishment of periodontal defects in all rats, the ligation was removed and 3 rats were scarified as negative control (group 1). The remaining animals were divided into three group based on treatment applied following mucoperiosteal flap surgery. Group 2 received saline treatment only, group 3 received doxycycline periostat (1.5 mg/kg/day) for 3 weeks, and group 4 received aminoguanidine (7.3 mmol/kg) for 3 weeks. The fasting glucose level was measured weekly post operatively. After 21 days all rats were sacrificed. Three anterior parts of the mandible of each group was prepared for histopathological examination and two parts were prepared for SEM.

RESULTS

Aminoguanidine treated group (group 4) showed statistically significant increased new bone formation, higher number of osteoblasts and decrease osteoclasts number, resorptive lacunae and existing inflammatory cell infiltration as compared to positive control group (group 2) (P<0.05). Doxycycline was also effective in reducing bone loss as documental by histopathological study.

CONCLUSION

The present study showed that aminoguanidine was significantly effective in reducing alveolar bone loss and can modify the detrimental effects of diabetes in alveolar bone resorption.

摘要

目的

氨基胍（盐酸胍基肼）是一种药物，通过对晚期糖基化终末产物（AGEs）积累的抑制活性，可预防糖尿病的许多典型全身性并发症，包括糖尿病性骨质减少。本研究的目的是使用传统组织病理学和扫描电子显微镜（SEM），评估氨基胍与强力霉素在减少糖尿病大鼠黏骨膜瓣术后牙槽骨吸收方面的有效性。

方法

本研究使用了27只雄性白化大鼠。在前下牙实验性诱导牙周缺损。所有大鼠通过静脉注射胰腺β细胞毒素一水合四氧嘧啶诱导糖尿病。在所有大鼠牙周缺损形成八周后，移除结扎线，处死3只大鼠作为阴性对照（第1组）。其余动物根据黏骨膜瓣手术后的治疗分为三组。第2组仅接受生理盐水治疗，第3组接受强力霉素牙周静息剂（1.5mg/kg/天）治疗3周，第4组接受氨基胍（7.3mmol/kg）治疗3周。术后每周测量空腹血糖水平。21天后处死所有大鼠。每组取下颌骨的三个前部进行组织病理学检查，取两个部分进行SEM检查。