Fukuda H, Yasuda H, Shimokawa S, Tamura M
Department of Cardiovascular Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Adv Exp Med Biol. 1989;248:567-73. doi: 10.1007/978-1-4684-5643-1_63.
The relationship between the energy state and intracellular oxygen concentration was established with cardiac tissue, where the former could be monitored by 31P-NMR and the latter by optical method for myoglobin absorption. The ratio of creatinephosphate to inorganic phosphate, PCr/Pi, started to fall at higher intracellular oxygen concentration than oxygen consumption (10IM). The pH shift measured by frequency shift of Pi paralleled the increase of lactate-release, of which half maximum was approximately 3IM. The critical oxygen concentrations differed nearly 5-fold when determined by energy state and oxygen consumption.
通过心脏组织建立了能量状态与细胞内氧浓度之间的关系，其中前者可通过31P-NMR监测，后者可通过肌红蛋白吸收的光学方法监测。磷酸肌酸与无机磷酸的比值PCr/Pi在高于耗氧量（10μM）的较高细胞内氧浓度时开始下降。通过Pi的频移测量的pH变化与乳酸释放的增加平行，其半数最大值约为3μM。当通过能量状态和耗氧量测定时，临界氧浓度相差近5倍。
