Taniguchi H, Shioaki Y, Daidoh T, Itoh A, Tsukuda N, Yamaguchi T, Sawai K, Takahashi T
First Dept. of Surgery, Kyoto Prefectural University of Medicine.
Gan To Kagaku Ryoho. 1989 Aug;16(8 Pt 2):3015-8.
As the model of an anti-cancer agent acting at DNA synthesizing phase, BrdU was infused for metastatic liver cancers into the portal vein and/or the hepatic artery. After administration, the liver tumors were resected, and immunohistochemical staining was performed using anti-BrdU monoclonal antibody. Two of 6 portal group tumors were stained. All 4 arterial and 3 arterio-portal group tumors were stained. Therefore, it was proved that metastatic liver tumors had both arterial and portal blood supply. But areas to which BrdU was delivered were only peripheral, and the inmost area was 2 mm from the tumor surface. After the intra-arterial infusion of BrdU suspended in lipiodol, the delivery of BrdU was highly enhanced and 4 of 6 tumors were stained even to the deepest areas. However, one tumor of which the major part was necrotic and one tumor which produced mutin were not stained at all. It was interesting that after intra-arterial administration of BrdU suspended in lipiodol, BrdU was also found in the cytoplasm of normal liver cells.
作为一种作用于DNA合成期的抗癌剂模型，将BrdU经门静脉和/或肝动脉注入转移性肝癌中。给药后，切除肝肿瘤，并使用抗BrdU单克隆抗体进行免疫组化染色。门静脉组的6个肿瘤中有2个被染色。动脉组的4个肿瘤和动门静脉组的3个肿瘤均被染色。因此，证明转移性肝肿瘤同时具有动脉和门静脉血供。但BrdU到达的区域仅为周边区域，最内部区域距离肿瘤表面2毫米。在将悬浮于碘油中的BrdU进行动脉内注射后，BrdU的递送显著增强，6个肿瘤中有4个甚至最深层区域也被染色。然而，一个大部分坏死的肿瘤和一个产生黏液的肿瘤完全未被染色。有趣的是，在将悬浮于碘油中的BrdU进行动脉内给药后，正常肝细胞的细胞质中也发现了BrdU。
