Kawata M, Kawahara Y, Araki S, Sunako M, Tsuda T, Fukuzaki H, Mizoguchi A, Takai Y
Department of Internal Medicine, Kobe University School of Medicine, Japan.
Biochem Biophys Res Commun. 1989 Sep 29;163(3):1418-27. doi: 10.1016/0006-291x(89)91137-6.
At least two GTP-binding proteins (G proteins) with Mr values of about 20,000 were extracted from bovine aortic smooth muscle membranes by sodium cholate. The most abundant G protein (22K G) was purified to near homogeneity by successive column chromatographies of Ultrogel AcA-44, phenyl-Sepharose CL-4B, hydroxyapatite and Mono Q HR5/5. 22K G showed kinetic and physical properties very similar to those of smg p21, a G protein recently isolated from bovine brain and human platelet membranes, having the same effector domain as ras p21s. Moreover, 22K G was recognized specifically by the anti-smg p21 antibody. These results indicate that the major G protein in bovine aortic smooth muscle membranes is smg p21.
用胆酸钠从牛主动脉平滑肌膜中提取出至少两种分子量约为20,000的GTP结合蛋白（G蛋白）。最丰富的G蛋白（22K G）通过连续的Ultrogel AcA - 44、苯基 - 琼脂糖CL - 4B、羟基磷灰石和Mono Q HR5/5柱色谱法纯化至接近均一。22K G表现出的动力学和物理性质与最近从牛脑和人血小板膜中分离出的G蛋白smg p21非常相似，其效应结构域与ras p21相同。此外，22K G能被抗smg p21抗体特异性识别。这些结果表明，牛主动脉平滑肌膜中的主要G蛋白是smg p21。
