Sherwood J B, Bard R, Bhargava M, Burns E R, Iqbal M A
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York.
Cancer Genet Cytogenet. 1989 Oct 1;42(1):35-42. doi: 10.1016/0165-4608(89)90005-8.
The cytogenetic, histologic, and electron microscopic studies of an adult patient with Wilms' tumor are presented. Wilms' tumor (nephroblastoma) is a common renal tumor of childhood but is extremely rare in people over 15 years old. The histologic analysis of the patient's tumor, including both light and electron microscopic analysis, indicated that this tumor satisfies the histologic criteria for an adult Wilms' tumor, namely, blastemic cells that are immature renal parenchymal cells, embryonic tubular structures, and a scanty stromal component consisting of loosely arranged spindle cells. The tumor showed several ultrastructural features characteristic of adult Wilms' tumor, namely, markedly elongated mitochondria, autophagic vacuoles, and intracytoplasmic filaments. Karyotypic analysis was performed on the patient's peripheral leukocytes and tumor cells. The leukocytes showed no significant increase in gaps and breaks, and the patient appears to have a normal male karyotype. Some interesting chromosomal anomalies were observed in the cultured tumor cells: at least one chromosome 13, both chromosomes 22, and the X chromosome are missing, three markers are present, and there is a possible deletion of 12p.
本文呈现了一位成年肾母细胞瘤患者的细胞遗传学、组织学及电子显微镜研究结果。肾母细胞瘤是儿童期常见的肾脏肿瘤，但在15岁以上人群中极为罕见。对该患者肿瘤进行的组织学分析，包括光镜和电镜分析，表明此肿瘤符合成人肾母细胞瘤的组织学标准，即幼稚肾实质细胞构成的母细胞成分、胚胎性管状结构以及由疏松排列的梭形细胞组成的少量间质成分。该肿瘤呈现出成人肾母细胞瘤的几个超微结构特征，即明显拉长的线粒体、自噬空泡和胞质内细丝。对患者外周血白细胞和肿瘤细胞进行了核型分析。白细胞的间隙和断裂未见明显增加，患者似乎具有正常男性核型。在培养的肿瘤细胞中观察到一些有趣的染色体异常：至少一条13号染色体、两条22号染色体和X染色体缺失，存在三个标记物，且可能存在12p缺失。
