Hernández-Rocamora Víctor M, García-Montañés Concepción, Rivas Germán
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), 28040, Madrid, Spain.
Curr Top Med Chem. 2014;14(23):2637-46. doi: 10.2174/1568026614666141215142951.
Nanodiscs are disc-like structures formed by two copies of a membrane scaffold protein, engineered from apolipoprotein A-I, surrounding a phospholipid mixture that can incorporate membrane proteins preserving their natural properties. They behave as soluble entities allowing the use of high-resolution structural techniques to determine the structural organization of the embedded membrane protein, and the use of solution biochemical-biophysical tools to measure its activity, assembly and interactions with other proteins in membranelike environments. In addition, nanodiscs are biocompatible which makes them an attractive technology to be used in therapy, drug discovery, and other biotechnological applications.
纳米圆盘是由载脂蛋白A-I改造而来的膜支架蛋白的两个拷贝形成的盘状结构，其围绕着一种磷脂混合物，该磷脂混合物可以整合膜蛋白并保持其天然特性。它们表现为可溶性实体，这使得可以使用高分辨率结构技术来确定嵌入膜蛋白的结构组织，并使用溶液生化-生物物理工具来测量其活性、组装以及在类膜环境中与其他蛋白质的相互作用。此外，纳米圆盘具有生物相容性，这使其成为一种有吸引力的技术，可用于治疗、药物发现和其他生物技术应用。
