Xu Li, Jin Qing-Dong, Gong Xi, Liu Hui, Zhou Rui-Xiang
Department of Physiology, Basic Medical College of Putian University, Putian 351100, China; Department of Human Anatomy and Histology and Embryology, Neurobiology Research Center, Fujian Medical University, Fuzhou 350004, China.
Sheng Li Xue Bao. 2014 Dec 25;66(6):723-9.
In order to investigate the role of melatonin in inhibiting the proliferation of murine gastric cancer and the underlying molecular mechanism, we performed an in vivo study by inoculating murine foregastric carcinoma (MFC) cells in mice, and then tumor-bearing mice were treated with different concentrations of melatonin (i.p.). The changes of Bcl-2, Bax, p21 and p53 expressions in tumor tissue were detected by using real-time fluorescence quantitative RT-PCR and Western blot. We found that: (1) melatonin resulted in reductions of tumor's volume and weight in the gastric cancer-bearing mice and thus showed anti-cancer effect; (2) melatonin reduced Bcl-2 expression, but increased the expression of Bax, p53 and p21 in tumor tissue. Our results suggest that melatonin could inhibit the growth of tumors in gastric cancer-bearing mice through accelerating the apoptosis of tumor cells.
为了研究褪黑素在抑制小鼠胃癌增殖中的作用及其潜在分子机制，我们通过将小鼠前胃癌（MFC）细胞接种到小鼠体内进行了一项体内研究，然后对荷瘤小鼠腹腔注射不同浓度的褪黑素进行处理。采用实时荧光定量逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测肿瘤组织中Bcl-2、Bax、p21和p53表达的变化。我们发现：（1）褪黑素可使荷胃癌小鼠的肿瘤体积和重量减小，从而显示出抗癌作用；（2）褪黑素降低了肿瘤组织中Bcl-2的表达，但增加了Bax、p53和p21的表达。我们的结果表明，褪黑素可通过加速肿瘤细胞凋亡来抑制荷胃癌小鼠肿瘤的生长。
