Vargas S J, Holden S N, Fall P M, Raisz L G
Department of Medicine, University of Connecticut Health Center Farmington 06032.
Endocrinology. 1989 Nov;125(5):2527-31. doi: 10.1210/endo-125-5-2527.
We examined the effects of synthetic human atrial natriuretic factor (human ANF 99-126) on adenylate cyclase activity, cAMP and cyclic GMP (cGMP) levels, bone resorption, collagen and DNA synthesis, and prostaglandin E2 (PGE2) production in fetal rat bone organ cultures. ANF (100 nM) inhibited PTH- and PGE2-stimulated cAMP production but had no effect on basal cAMP production in 21-day fetal rat calvaria. ANF increased cGMP levels, and this was not affected by PTH. ANF (10 nM) partially inhibited bone resorption stimulated by PGE2 but had no effect on control or PTH-stimulated resorption in 19-day fetal rat long bones. ANF had no effect on collagen and DNA synthesis or PGE2 production and did not alter responses to PTH or PGE2 in the fetal rat calvaria. Thus, ANF has no major direct effect on bone resorption or formation, but it is possible that ANF modulates the local regulatory function of PGE2 in bone.
我们研究了合成的人心房钠尿肽（人ANF 99 - 126）对胎鼠骨器官培养物中腺苷酸环化酶活性、环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平、骨吸收、胶原蛋白和DNA合成以及前列腺素E2（PGE2）产生的影响。在21天胎鼠的颅骨中，ANF（100 nM）抑制甲状旁腺激素（PTH）和PGE2刺激的cAMP产生，但对基础cAMP产生无影响。ANF增加cGMP水平，且不受PTH影响。在19天胎鼠的长骨中，ANF（10 nM）部分抑制PGE2刺激的骨吸收，但对对照或PTH刺激的骨吸收无影响。ANF对胶原蛋白和DNA合成或PGE2产生无影响，且不改变胎鼠颅骨对PTH或PGE2的反应。因此，ANF对骨吸收或形成无主要直接影响，但ANF可能调节PGE2在骨中的局部调节功能。
