苯的自由基化学与生物化学机制。
Mechanisms of free radical chemistry and biochemistry of benzene.
作者信息
Karam L R, Simic M G
机构信息
Center for Radiation Research, National Bureau of Standards, Gaithersburg, MD 20899.
出版信息
Environ Health Perspect. 1989 Jul;82:37-41. doi: 10.1289/ehp.898237.
omicron-Tyrosine (omicron-Tyr) was used as a specific biomarker for .OH radicals generated in biosystems. Specificity of omicron-Tyr as an .OH biomarker was based on previous studies in systems exposed to ionizing radiations. Fresh muscle tissue incubated with benzene for 1 hr at 38 degrees C exhibits formation of omicron-Tyr as seen in the cases of ethanol- and carbon tetrachloride-exposed systems. Gas chromatography/mass spectrometry selective ion monitoring measurements of omicron-Tyr yields in chicken breast muscle incubated with water or benzene indicate levels of less than 0.1 ppm and 3.0 +/- 0.5 ppm of omicron-Tyr, respectively. Formation of .OH is presumed to originate via a Haber-Weiss reaction of H2O2 with Fe(II) preceded by the formation of .O2- and H2O2 from distorted mitochondria.
奥密克戎-酪氨酸（omicron-Tyr）被用作生物系统中产生的羟基自由基（·OH）的特异性生物标志物。奥密克戎-酪氨酸作为·OH生物标志物的特异性基于先前在暴露于电离辐射的系统中的研究。新鲜肌肉组织在38℃下与苯孵育1小时后，会出现奥密克戎-酪氨酸的形成，这与乙醇和四氯化碳暴露系统的情况类似。气相色谱/质谱选择性离子监测对用水或苯孵育的鸡胸肉中奥密克戎-酪氨酸产量的测量表明，其水平分别低于0.1 ppm和3.0±0.5 ppm。据推测，·OH的形成是通过H2O2与Fe(II)的哈伯-维伊斯反应产生的，在此之前，扭曲的线粒体先形成超氧阴离子（·O2-）和H2O2。
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