大鼠C细胞中胞质游离钙的节律性振荡

Rhythmic oscillations of cytosolic free calcium in rat C-cells.

作者信息

Eckert R W, Scherübl H, Petzelt C, Raue F, Ziegler R

机构信息

Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.

出版信息

Mol Cell Endocrinol. 1989 Jul;64(2):267-70. doi: 10.1016/0303-7207(89)90154-8.

DOI:10.1016/0303-7207(89)90154-8

PMID:2551759

Abstract

The relationship between stimulation of single C-cells (rMTC-6-23 cell line) with extracellular calcium, glucagon or 8-bromo-cAMP and fluctuations of intracellular free calcium concentration was studied. After pretreatment of rMTC cells with either 1 microM glucagon (30-60 min) or 1 mM 8-bromo-cAMP (5 min) [Ca2+]i started to oscillate when extracellular calcium was raised to 3 mM. These fluctuations in [Ca2+]i could be stopped by chelating the external calcium with EGTA or by adding calcium channel blockers. The voltage-dependent calcium channels in the plasma membrane seem to play a major role in maintaining the oscillations of [Ca2+]i.

摘要

研究了用细胞外钙、胰高血糖素或8-溴环磷酸腺苷刺激单个C细胞（rMTC-6-23细胞系）与细胞内游离钙浓度波动之间的关系。在用1微摩尔胰高血糖素（30-60分钟）或1毫摩尔8-溴环磷酸腺苷（5分钟）对rMTC细胞进行预处理后，当细胞外钙升高到3毫摩尔时，[Ca2+]i开始振荡。通过用乙二醇双四乙酸螯合细胞外钙或添加钙通道阻滞剂，可以阻止[Ca2+]i的这些波动。质膜中电压依赖性钙通道似乎在维持[Ca2+]i振荡中起主要作用。