豆科根瘤菌自由生活细菌和类菌体中共生质粒DNA的比较研究

Comparative study of the symbiotic plasmid DNA in free living bacteria and bacteroids of Rhizobium leguminosarum.

作者信息

Vierny C, Iaccarino M

机构信息

International Institute of Genetics and Biophysics, CNR, Naples, Italy.

出版信息

FEMS Microbiol Lett. 1989 Jul 1;51(1):15-19. doi: 10.1016/0378-1097(89)90069-4.

DOI:10.1016/0378-1097(89)90069-4

PMID:2551770

Abstract

The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed in bacteroids as compared to bacteria. No rearrangements of the symbiotically expressed pSym borne genes were detected in bacteroids.

摘要

通过使用合适的探针进行杂交实验，对豌豆根瘤菌生物变种RCR1001菌株类菌体中存在的共生质粒（pSym）DNA与自由生活细菌中存在的pSym DNA进行了定性和定量比较。与细菌相比，观察到类菌体中pSym DNA的相对量有所减少。在类菌体中未检测到共生表达的pSym携带基因的重排。