Oliveira Juliana Santos, Raucci Neto Walter, Faria Natália Spadine de, Fernandes Fernanda Silva, Miranda Carlos Eduardo Saraiva, Abi Rached-Junior Fuad Jacob
Dental School, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
Braz Dent J. 2014 Sep-Oct;25(5):409-15. doi: 10.1590/0103-6440201302433.
Chemical solutions play important roles in endodontic treatment and promote ultrastructural changes in dentin surface. The aim of this study was to quantify root canal roughness at different concentrations of calcium hypochlorite (Ca(OCl)2) and sodium hypochlorite (NaOCl) by confocal laser scanning microscopy (CLSM). Fifty-two human mandibular premolars were sectioned and randomly organized into thirteen groups (n=8): saline (control); 1%, 2.5% and 5% NaOCl; 1%, 2.5% and 5% Ca(OCl)2; the hypochlorite groups were further divided into with or without EDTA. The chlorine concentrations of the different solutions were measured by iodine titration (%). The superficial roughness (Sa) was quantified by CLSM. Ca(OCl)2 presented substantial decrease in chlorine concentration that differed from the package indication, but without compromising the dentin ultrastructure changes. There were no significant differences in dentin roughness between Ca(OCl)2 or NaOCl at all studied concentrations. The combination with EDTA provided similar roughness values among the solutions (p>0.05). The 5% Ca(OCl)2 and NaOCl solutions significantly increased dentin roughness and did not differ from the EDTA association (p>0.05). Ca(OCl)2 promoted similar dentin roughness as the NaOCl at the same concentrations and combined with EDTA. It may be concluded that Ca(OCl)2 modified the root canal dentin roughness similarly to NaOCl, at the same concentrations and EDTA combinations used in this study. Ca(OCl)2 and NaOCl, both at 5%, significantly altered dentin roughness, overcoming EDTA association, thus Ca(OCl)2 concentrations ranging from 1% to 2.5% may be suitable solutions for root canal irrigation protocols.
化学溶液在根管治疗中发挥着重要作用，并促进牙本质表面的超微结构变化。本研究的目的是通过共聚焦激光扫描显微镜（CLSM）定量不同浓度的次氯酸钙（Ca(OCl)₂）和次氯酸钠（NaOCl）下的根管粗糙度。将52颗人类下颌前磨牙进行切片，并随机分为13组（n = 8）：生理盐水（对照组）；1%、2.5%和5%的NaOCl；1%、2.5%和5%的Ca(OCl)₂；次氯酸盐组进一步分为添加或不添加乙二胺四乙酸（EDTA）。通过碘滴定法（%）测量不同溶液的氯浓度。通过CLSM定量表面粗糙度（Sa）。Ca(OCl)₂的氯浓度显著降低，与包装说明不同，但不影响牙本质超微结构变化。在所有研究浓度下，Ca(OCl)₂或NaOCl之间的牙本质粗糙度没有显著差异。与EDTA联合使用时，各溶液的粗糙度值相似（p>0.05）。5%的Ca(OCl)₂和NaOCl溶液显著增加了牙本质粗糙度，与EDTA联合使用时无差异（p>0.05）。在相同浓度下，Ca(OCl)₂与EDTA联合使用时促进的牙本质粗糙度与NaOCl相似。可以得出结论，在本研究中使用的相同浓度和EDTA组合下，Ca(OCl)₂改变根管牙本质粗糙度的方式与NaOCl相似。5%的Ca(OCl)₂和NaOCl均显著改变了牙本质粗糙度，超过了与EDTA联合使用的效果，因此1%至2.5%的Ca(OCl)₂浓度可能是根管冲洗方案的合适溶液。
