Aziret Mehmet, Irkorucu Oktay, Reyhan Enver, Erdem Hasan, Das Koray, Ozkara Selvinaz, Surmelioglu Ali, Sozen Selim, Bali Ilhan, Cetinkunar Sulleyman, Deger Kamuran Cumhur
Kars State HospitalDepartment of General SurgeryKars, Department of General Surgery, Kars State Hospital, TurkeyKars, Turkey.
Department of General Surgery, Adana Adana Numune Training and Research Hospital, Adana, Turkey.
Clinics (Sao Paulo). 2014 Nov;69(11):763-9. doi: 10.6061/clinics/2014(11)10.
Vardenafil enhances dilatation of vascular smooth muscle and inhibits platelet aggregation. The purpose of this study was to evaluate the clinical effects of vardenafil and pentoxifylline administration in an experimental model of ischemic colitis.
Forty female Wistar albino rats weighing 250-300 g were randomized into five experimental groups (each with n = 8) as follows:1) a sham group subjected to a sham surgical procedure and administered only tap water; 2) a control group subjected to a standardized surgical procedure to induce ischemic colitis and administered only tap water; 3) and 4) treatment groups subjected to surgical induction of ischemic colitis followed by the postoperative administration of 5 mg/kg or 10 mg/kg vardenafil, respectively; and 5) a treatment group subjected to surgical induction of ischemic colitis followed by postoperative administration of pentoxifylline at 50 mg/kg/day per day as a single dose for a 3-day period. All animals were sacrificed at 72 h post-surgery and subjected to relaparotomy. We scored the macroscopically visible damage, measured the ischemic area and scored histopathology to determine the severity of ischemia. Tissue malondialdehyde levels were also quantified.
The mean Gomella ischemic areas were 63.3 mm2 in the control group; 3.4 and 9.6 mm2 in the vardenafil 5 and vardenafil 10 groups, respectively; and 3.4 mm2 in the pentoxifylline group (p = 0.0001). The mean malondialdehyde values were 63.7 nmol/g in the control group; 25.3 and 25.6 nmol/g in the vardenafil 5 and vardenafil 10 groups, respectively; and 22.8 nmol/g in the pentoxifylline group (p = 0.0001).
Our findings indicate that vardenafil and pentoxifylline are effective treatment options in an animal model of ischemic colitis. The positive clinical effects produced by these drugs are likely due to their influence on the hemodynamics associated with vascular smooth muscle and platelet functions.
伐地那非可增强血管平滑肌舒张并抑制血小板聚集。本研究旨在评估伐地那非和己酮可可碱在缺血性结肠炎实验模型中的临床效果。
将40只体重250 - 300克的雌性Wistar白化大鼠随机分为五个实验组（每组n = 8），如下：1）假手术组，接受假手术操作，仅给予自来水；2）对照组，接受标准化手术操作以诱导缺血性结肠炎，仅给予自来水；3）和4）治疗组，接受手术诱导缺血性结肠炎，术后分别给予5毫克/千克或10毫克/千克伐地那非；5）治疗组，接受手术诱导缺血性结肠炎，术后以50毫克/千克/天的剂量单次给予己酮可可碱，持续3天。所有动物在术后72小时处死并进行再次剖腹术。我们对肉眼可见的损伤进行评分，测量缺血面积并对组织病理学进行评分以确定缺血严重程度。还对组织丙二醛水平进行了定量分析。
对照组的平均戈梅拉缺血面积为63.3平方毫米；伐地那非5毫克/千克组和伐地那非10毫克/千克组分别为3.4平方毫米和9.6平方毫米；己酮可可碱组为3.4平方毫米（p = 0.0001）。对照组的平均丙二醛值为63.7纳摩尔/克；伐地那非5毫克/千克组和伐地那非10毫克/千克组分别为25.3纳摩尔/克和25.6纳摩尔/克；己酮可可碱组为22.8纳摩尔/克（p = 0.0001）。
我们的研究结果表明，伐地那非和己酮可可碱在缺血性结肠炎动物模型中是有效的治疗选择。这些药物产生的积极临床效果可能归因于它们对与血管平滑肌和血小板功能相关的血流动力学的影响。
