Bradley J L, Piatak M, Lane J A, McGuire P M
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville.
Int J Pept Protein Res. 1989 Jul;34(1):2-5. doi: 10.1111/j.1399-3011.1989.tb00999.x.
Successful immunotoxin therapy may depend upon reduction of the size of the components in order to decrease antigenicity and rate of clearance. In initial attempts to modify the A chain of ricin by deletion analyses within a prokaryotic expression system, coding sequences were modified by the insertion of unique restriction endonuclease sites and by the removal of 22 codons near the 5' terminus. The work presented here examines the expression, solubility, and activity of these mutant proteins and demonstrates that while amino acid residues may be altered in this region, the deletion of residues 19 through 40 yields an insoluble and inactive toxin molecule.
成功的免疫毒素疗法可能取决于减小其组分的大小，以降低抗原性和清除率。在最初尝试通过原核表达系统中的缺失分析来修饰蓖麻毒素A链时，编码序列通过插入独特的限制性内切酶位点以及去除5'末端附近的22个密码子进行了修饰。本文展示的研究考察了这些突变蛋白的表达、溶解性和活性，并证明虽然该区域的氨基酸残基可能会发生改变，但删除19至40位残基会产生不溶性且无活性的毒素分子。
