Lisitsyna O M, Musinova Ia R, Shubina M Ia, Poliakov V Ia, Sheval' E V
Izv Akad Nauk Ser Biol. 2013 Nov-Dec(6):750-3.
Interphase prenucleolar bodies are globular structures which accumulate in large numbers in the nucleoplasm of cultivated cells after hypotonic treatment and subsequent return to isotonic conditions; detailed studies of the role of these structures in the recovery of the nucleolus have not yet been performed. The limited mobility of interphase pronucleoli within the nucleus has been demonstrated. Exchange of the major nucleolar protein B23 between prenucleolar bodies and the surrounding nucleoplasm, rather than stable binding of this protein to the prenucleolar bodies, has been demonstrated using fluorescence recovery after photobleaching method. Gradual accumulation of B23 in the recovering nucleolus with concomitant disappearance of prenucleolar bodies has been demonstrated.
间期前核仁体是球状结构，在低渗处理后大量积聚在培养细胞的核质中，随后恢复到等渗条件；尚未对这些结构在核仁恢复中的作用进行详细研究。已证明间期前核仁在核内的移动性有限。使用光漂白后荧光恢复方法已证明，前核仁体与周围核质之间存在主要核仁蛋白B23的交换，而不是该蛋白与前核仁体的稳定结合。已证明B23在恢复的核仁中逐渐积累，同时前核仁体消失。
