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[甲草胺和Cd2+对斜生栅藻光合作用的影响]

[Influence of S-metolachlor and Cd2+ on photosynthesis of Scenedesmus obliquus].

作者信息

Chen Cai-dong, Hu Xiao-na, Zhang Xiao-qiang, Liu Hui-jun

出版信息

Huan Jing Ke Xue. 2014 Sep;35(9):3498-503.

Abstract

The single and combined effects of Cd2+ and S-metolachlor on the chlorophyll content and chlorophyll fluorescence parameters of Scenedesmus obliquus were studied by using standard toxic testing method. Both Cd2+ and S-metolachlor had effects on the chlorophyll content and fluorescence parameters such as F0, FV/Fm, FV/F0, Y( II), qP, NPQ and rETR after 96 h-exposure, showing that Cd2+ and S-metolachlor damaged the PS II in algae, inhibited the primary reaction of photosynthesis, stopped the process of photosynthetic electron transport, and destroyed its ability of heat dissipation. The effects of Cd2 + on the chlorophyll content and fluorescence parameters were greater than those of S-metolachlor, and the effects increased with the increasing concentration. The average drop of Y( II ) was 62. 5% in the control group when the light intensity was 231 μmol (m2.s) -1 , and it was 68. 0% , 82. 5% and 100% respectively in Cd2+ -treated groups, and 66. 1% , 72. 1% and 79.6% respectively in S-metolachlor-treated group with the increasing concentration. The combined effects of Cd2+ and S-metolachlor on the chlorophyll fluorescence parameters were mainly due to the impacts of Cd2+.

摘要

采用标准毒性测试方法,研究了镉离子(Cd2+)和乙草胺对斜生栅藻叶绿素含量及叶绿素荧光参数的单一和联合效应。暴露96小时后,Cd2+和乙草胺均对叶绿素含量以及诸如F0、FV/Fm、FV/F0、Y(II)、qP、NPQ和rETR等荧光参数产生影响,表明Cd2+和乙草胺破坏了藻类中的光系统II,抑制了光合作用的原初反应,阻断了光合电子传递过程,并破坏了其散热能力。Cd2+对叶绿素含量和荧光参数的影响大于乙草胺,且影响程度随浓度增加而增大。当光强为231 μmol(m2·s)-1时,对照组Y(II)的平均降幅为62.5%,Cd2+处理组随浓度增加分别为68.0%、82.5%和100%,乙草胺处理组分别为66.1%、72.1%和79.6%。Cd2+和乙草胺对叶绿素荧光参数的联合效应主要归因于Cd2+的影响。

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