使用设计的肽核酸肽，基于蛋白酶活性对富含鸟嘌呤的DNA二级结构进行调控。

Control of guanine-rich DNA secondary structures depending on the protease activity using a designed PNA peptide.

作者信息

Usui Kenji, Okada Arisa, Kobayashi Keita, Sugimoto Naoki

机构信息

FIRST (Faculty of Frontiers of Innovative Research in Science and Technology), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.

出版信息

Org Biomol Chem. 2015 Feb 21;13(7):2022-5. doi: 10.1039/c4ob02535k.

DOI:10.1039/c4ob02535k

PMID:25519192

Abstract

We constructed a regulation system for DNA secondary structure formation of G-rich sequences using a designed PNA peptide exhibiting an on-to-off switching functionality, depending on the protease activity. This study introduces the new concept of a simple and powerful system for regulating quadruplex-related important biological events.

摘要

我们利用一种设计的肽核酸（PNA）肽构建了一个用于富含鸟嘌呤（G）序列的DNA二级结构形成的调控系统，该肽根据蛋白酶活性呈现开-关切换功能。本研究引入了一个用于调控与四链体相关的重要生物学事件的简单而强大的系统的新概念。