通过高压液相色谱（HPLC）监测马心细胞色素c的胃蛋白酶和胰蛋白酶水解来研究血红素八肽（微过氧化物酶-8；MP-8）的形成动力学。
Kinetics of heme octapeptide (microperoxidase-8; MP-8) formation studied by high-pressure liquid chromatography (HPLC) monitoring of the peptic and tryptic hydrolysis of horse heart cytochrome-c.
作者信息
Adams P A, Byfield M P, Goold R D, Thumser A E
机构信息
Department of Chemical Pathology, University of Cape Town Medical School, Republic of South Africa.
出版信息
J Inorg Biochem. 1989 Sep;37(1):55-9. doi: 10.1016/0162-0134(89)80029-7.
PMID:2552013
Abstract
The kinetics of the sequential peptic and tryptic hydrolysis of cytochrome-c to give the heme-peptides microperoxidase-11 (MP-11) and -8 (MP-8), respectively, has been investigated by high performance liquid chromatography (HPLC), and we demonstrate that MP-8 can be prepared from cytochrome-c to the point of lyophilization within 4 hr.
摘要
通过高效液相色谱法（HPLC）研究了细胞色素c依次经胃蛋白酶和胰蛋白酶水解分别生成血红素肽微过氧化物酶-11（MP-11）和-8（MP-8）的动力学，并且我们证明了MP-8可以在4小时内从细胞色素c制备至冻干阶段。
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