Matsumoto Kazumasa, Ikeda Masaomi, Matsumoto Toshihide, Nagashio Ryo, Nishimori Takanori, Tomonaga Takeshi, Nomura Fumio, Sato Yuichi, Kitasato Hidero, Iwamura Masatsugu
Departments of Urology, School of Medicine, Kitasato University, Japan E-mail :
Asian Pac J Cancer Prev. 2014;15(22):9927-31. doi: 10.7314/apjcp.2014.15.22.9927.
The objectives of this study were to examine serum periplakin expression in patients with urothelial carcinoma of the urinary bladder and in normal controls, and to examine relationships with clinicopathological findings. Detection of serum periplakin was performed in 50 patients and 30 normal controls with anti-periplakin antibodies using the automatic dot blot system, and a micro-dot blot array with a 256 solid-pin system. Levels in patients with urothelial carcinoma of the urinary bladder were significantly lower than those in normal controls (0.31 and 5.68, respectively; p<0.0001). The area under the receiver-operator curve level for urothelial carcinoma of the urinary bladder was 0.845. The sensitivity and specificity, using a cut-off point of 4.045, were 83.7% and 73.3%, respectively. In addition, serum periplakin levels were significantly higher in patients with muscle-invasive cancer than in those with nonmuscle-invasive cancer (P=0.03). In multivariate Cox proportional hazards regression analysis, none of the clinicopathological factors was associated with an increased risk for progression and cancer-specific survival. Examination of the serum periplakin level may play a role as a non- invasive diagnostic modality to aid urine cytology and cystoscopy.
本研究的目的是检测膀胱尿路上皮癌患者和正常对照者血清中周斑蛋白的表达，并研究其与临床病理特征的关系。采用自动点杂交系统和具有256个实心针系统的微点杂交阵列，用抗周斑蛋白抗体对50例患者和30例正常对照者进行血清周斑蛋白检测。膀胱尿路上皮癌患者的血清周斑蛋白水平显著低于正常对照者（分别为0.31和5.68；p<0.0001）。膀胱尿路上皮癌的受试者工作特征曲线下面积为0.845。以4.045为截断点时，敏感性和特异性分别为83.7%和73.3%。此外，肌层浸润性癌患者的血清周斑蛋白水平显著高于非肌层浸润性癌患者（P=0.03）。在多因素Cox比例风险回归分析中，没有临床病理因素与进展风险和癌症特异性生存风险增加相关。检测血清周斑蛋白水平可能作为一种非侵入性诊断方法，辅助尿液细胞学检查和膀胱镜检查。
