微小RNA-543通过靶向PAQR3在肝细胞癌中发挥癌基因作用。
MicroRNA-543 acts as an oncogene by targeting PAQR3 in hepatocellular carcinoma.
作者信息
Yu Lei, Zhou Luting, Cheng Yu, Sun Lei, Fan Jian, Liang Jinlong, Guo Mujie, Liu Ning, Zhu Liying
机构信息
Department of Infectious Disease, The Fourth Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
Department of Pathology, General Hospital of Jinan Military Command Jinan 250000, Shandong, China.
出版信息
Am J Cancer Res. 2014 Nov 19;4(6):897-906. eCollection 2014.
Abstract
MicroRNAs (miRNAs) are small, non-coding RNAs that can act as oncogenes or tumor suppressor genes in human cancer. Increasing evidences indicate that deregulation of miRNAs contributes to the hepatocarcinogenesis. In this study, we demonstrated that the levels of miR-543 were dramatically increased in clinical hepatocellular carcinoma (HCC) tissues and cell lines. Moreover, forced expression of miR-543 promoted the proliferative and invasive potential of HepG2. We also identified PAQR3 as a direct target gene for miR-543 using a fluorescent reporter assay and western blot. The levels of PAQR3 were dramatically decreased in clinical hepatocellular carcinoma (HCC) tissues and cell lines. The mRNA levels of PAQR3 were inversely correlated with the miR-543 expression level.Thus, our finding provides a new insight into the mechanism of hepatocarcinogenesis, indicating a therapeutic potential of miR-543 in the treatment of HCC.
摘要
微小RNA（miRNA）是一类小的非编码RNA，在人类癌症中可作为癌基因或肿瘤抑制基因发挥作用。越来越多的证据表明，miRNA失调与肝癌发生有关。在本研究中，我们证明miR-543在临床肝细胞癌（HCC）组织和细胞系中的水平显著升高。此外，强制表达miR-543可促进HepG2的增殖和侵袭能力。我们还通过荧光报告基因检测和蛋白质印迹法确定PAQR3是miR-543的直接靶基因。PAQR3在临床肝细胞癌（HCC）组织和细胞系中的水平显著降低。PAQR3的mRNA水平与miR-543表达水平呈负相关。因此，我们的发现为肝癌发生机制提供了新的见解，表明miR-543在HCC治疗中具有治疗潜力。
相似文献
1
MicroRNA-543 acts as an oncogene by targeting PAQR3 in hepatocellular carcinoma.微小RNA-543通过靶向PAQR3在肝细胞癌中发挥癌基因作用。
Am J Cancer Res. 2014 Nov 19;4(6):897-906. eCollection 2014.
2
MicroRNA-520e suppresses growth of hepatoma cells by targeting the NF-κB-inducing kinase (NIK).微小 RNA-520e 通过靶向 NF-κB 诱导激酶 (NIK) 抑制肝癌细胞的生长。
Oncogene. 2012 Aug 2;31(31):3607-20. doi: 10.1038/onc.2011.523. Epub 2011 Nov 21.
3
MicroRNA-101 inhibits human hepatocellular carcinoma progression through EZH2 downregulation and increased cytostatic drug sensitivity.微小 RNA-101 通过下调 EZH2 并增加细胞抑制性药物敏感性抑制人肝癌进展。
J Hepatol. 2014 Mar;60(3):590-8. doi: 10.1016/j.jhep.2013.10.028. Epub 2013 Nov 6.
4
MicroRNA-195 acts as a tumor suppressor by directly targeting Wnt3a in HepG2 hepatocellular carcinoma cells.微小RNA-195通过直接靶向HepG2肝癌细胞中的Wnt3a发挥肿瘤抑制作用。
Mol Med Rep. 2014 Nov;10(5):2643-8. doi: 10.3892/mmr.2014.2526. Epub 2014 Aug 28.
5
[The effects of microRNA-7 on proliferation and invasion of hepatocellular carcinoma HepG2 cells].[微小RNA-7对肝癌HepG2细胞增殖和侵袭的影响]
Zhonghua Zhong Liu Za Zhi. 2018 Jun 23;40(6):406-411. doi: 10.3760/cma.j.issn.0253-3766.2018.06.002.
6
miR-10b exerts oncogenic activity in human hepatocellular carcinoma cells by targeting expression of CUB and sushi multiple domains 1 (CSMD1).微小RNA-10b通过靶向CUB和寿司多结构域蛋白1（CSMD1）的表达在人肝癌细胞中发挥致癌活性。
BMC Cancer. 2016 Oct 18;16(1):806. doi: 10.1186/s12885-016-2801-4.
7
A single nucleotide variant in microRNA-1269a promotes the occurrence and process of hepatocellular carcinoma by targeting to oncogenes SPATS2L and LRP6.微小RNA-1269a中的一个单核苷酸变体通过靶向癌基因SPATS2L和LRP6促进肝细胞癌的发生和发展。
Bull Cancer. 2017 Apr;104(4):311-320. doi: 10.1016/j.bulcan.2016.11.021. Epub 2017 Jan 9.
8
Identification a novel tumor-suppressive hsa-miR-599 regulates cells proliferation, migration and invasion by targeting oncogenic MYC in hepatocellular carcinoma.鉴定一种新型肿瘤抑制性人源 microRNA-599 通过靶向致癌基因 MYC 调控肝癌细胞的增殖、迁移和侵袭。
Am J Transl Res. 2016 Jun 15;8(6):2575-84. eCollection 2016.
9
MicroRNA-592 targets DEK oncogene and suppresses cell growth in the hepatocellular carcinoma cell line HepG2.微小RNA-592靶向DEK癌基因并抑制肝癌细胞系HepG2中的细胞生长。
Int J Clin Exp Pathol. 2015 Oct 1;8(10):12455-63. eCollection 2015.
10
Long non-coding RNA PTTG3P functions as an oncogene by sponging miR-383 and up-regulating CCND1 and PARP2 in hepatocellular carcinoma.长链非编码 RNA PTTG3P 通过海绵吸附 miR-383 并上调 HCC 中的 CCND1 和 PARP2 发挥癌基因作用。
BMC Cancer. 2019 Jul 24;19(1):731. doi: 10.1186/s12885-019-5936-2.
引用本文的文献
1
The Emerging Roles and Mechanisms of PAQR3 in Human Cancer: Pathophysiology and Therapeutic Implications.PAQR3在人类癌症中的新兴作用和机制：病理生理学及治疗意义
Int J Gen Med. 2023 Sep 22;16:4321-4328. doi: 10.2147/IJGM.S422523. eCollection 2023.
2
Long noncoding RNA SNHG6 promotes the malignant phenotypes of ovarian cancer cells via miR-543/YAP1 pathway.长链非编码RNA SNHG6通过miR-543/YAP1途径促进卵巢癌细胞的恶性表型。
Heliyon. 2023 May 17;9(5):e16291. doi: 10.1016/j.heliyon.2023.e16291. eCollection 2023 May.
3
Retraction: MicroRNA-424 Is Down-Regulated in Hepatocellular Carcinoma and Suppresses Cell Migration and Invasion through c-Myb.撤稿声明：微小RNA-424在肝细胞癌中表达下调并通过c-Myb抑制细胞迁移和侵袭。
PLoS One. 2022 Aug 31;17(8):e0274145. doi: 10.1371/journal.pone.0274145. eCollection 2022.
4
LncRNA MEG3 inhibits the development of nasopharyngeal carcinoma by sponging miR-543 targeting KLF4.长链非编码RNA MEG3通过靶向KLF4的海绵吸附miR-543抑制鼻咽癌的发展。
Transl Cancer Res. 2020 Feb;9(2):958-971. doi: 10.21037/tcr.2019.12.41.
5
MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-β-Treated Endometrial Stromal Cells via the MAPK and Wnt/β-Catenin Signaling Pathways.miR-543 通过 MAPK 和 Wnt/β-连环蛋白信号通路抑制 TGF-β 处理的子宫内膜基质细胞的迁移和上皮间质转化。
Pathol Oncol Res. 2021 Apr 29;27:1609761. doi: 10.3389/pore.2021.1609761. eCollection 2021.
6
MicroRNA-543 inhibits the proliferation, migration, invasion, and epithelial-mesenchymal transition of triple-negative breast cancer cells via down-regulation of ACTL6A gene.微小 RNA-543 通过下调 ACTL6A 基因抑制三阴性乳腺癌细胞的增殖、迁移、侵袭和上皮间质转化。
Clin Transl Oncol. 2022 Jan;24(1):84-92. doi: 10.1007/s12094-021-02672-z. Epub 2021 Jun 28.
7
LncRNA SNHG6 promotes breast cancer progression and epithelial-mesenchymal transition via miR-543/LAMC1 axis.长链非编码 RNA SNHG6 通过 miR-543/LAMC1 轴促进乳腺癌进展和上皮-间充质转化。
Breast Cancer Res Treat. 2021 Jul;188(1):1-14. doi: 10.1007/s10549-021-06190-y. Epub 2021 Mar 29.
8
MiR-543/Numb promotes proliferation, metastasis, and stem-like cell traits of prostate cancer cells.微小RNA-543/麻木蛋白促进前列腺癌细胞的增殖、转移及干细胞样特性。
Am J Transl Res. 2021 Feb 15;13(2):617-631. eCollection 2021.
9
Circular RNA circARPP21 Acts as a Sponge of miR-543 to Suppress Hepatocellular Carcinoma by Regulating LIFR.环状RNA circARPP21作为miR-543的海绵，通过调控白血病抑制因子受体（LIFR）来抑制肝细胞癌。
Onco Targets Ther. 2021 Feb 5;14:879-890. doi: 10.2147/OTT.S283026. eCollection 2021.
10
MiR-21 Is Required for the Epithelial-Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells.miR-21 在 MDA-MB-231 乳腺癌细胞上皮间质转化中起关键作用。
Int J Mol Sci. 2021 Feb 4;22(4):1557. doi: 10.3390/ijms22041557.
本文引用的文献
1
A Golgi-specific protein PAQR3 is closely associated with the progression, metastasis and prognosis of human gastric cancers.一种高尔基体特异性蛋白 PAQR3 与人胃癌的进展、转移和预后密切相关。
Ann Oncol. 2014 Jul;25(7):1363-1372. doi: 10.1093/annonc/mdu168. Epub 2014 May 5.
2
MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression.MicroRNA-543 通过靶向 FAK 和 TWIST1 的表达抑制子宫内膜癌细胞的致癌性。
Arch Gynecol Obstet. 2014 Sep;290(3):533-41. doi: 10.1007/s00404-014-3219-3. Epub 2014 Apr 4.
3
miR-494 acts as an anti-oncogene in gastric carcinoma by targeting c-myc.miR-494通过靶向c-myc在胃癌中发挥抑癌基因的作用。
J Gastroenterol Hepatol. 2014;29(7):1427-34. doi: 10.1111/jgh.12558.
4
MicroRNA-545 suppresses cell proliferation by targeting cyclin D1 and CDK4 in lung cancer cells.MicroRNA-545 通过靶向肺癌细胞中的细胞周期蛋白 D1 和 CDK4 抑制细胞增殖。
PLoS One. 2014 Feb 5;9(2):e88022. doi: 10.1371/journal.pone.0088022. eCollection 2014.
5
MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1.微小 RNA-490-3p 通过靶向 CCND1 抑制 A549 肺癌细胞的增殖。
Biochem Biophys Res Commun. 2014 Jan 31;444(1):104-8. doi: 10.1016/j.bbrc.2014.01.020. Epub 2014 Jan 16.
6
MicroRNA-10b promotes nucleus pulposus cell proliferation through RhoC-Akt pathway by targeting HOXD10 in intervetebral disc degeneration.微小 RNA-10b 通过靶向椎间盘退变中 HOXD10 促进核内突细胞增殖的 RhoC-Akt 通路。
PLoS One. 2013 Dec 20;8(12):e83080. doi: 10.1371/journal.pone.0083080. eCollection 2013.
7
PAQR3 has modulatory roles in obesity, energy metabolism, and leptin signaling.PAQR3 在肥胖、能量代谢和瘦素信号中具有调节作用。
Endocrinology. 2013 Dec;154(12):4525-35. doi: 10.1210/en.2013-1633. Epub 2013 Sep 13.
8
miR-335 inhibits small cell lung cancer bone metastases via IGF-IR and RANKL pathways.miR-335 通过 IGF-IR 和 RANKL 通路抑制小细胞肺癌骨转移。
Mol Cancer Res. 2014 Jan;12(1):101-10. doi: 10.1158/1541-7786.MCR-13-0136. Epub 2013 Aug 21.
9
miRNA-218 inhibits osteosarcoma cell migration and invasion by down-regulating of TIAM1, MMP2 and MMP9.微小RNA-218通过下调TIAM1、基质金属蛋白酶2和基质金属蛋白酶9来抑制骨肉瘤细胞的迁移和侵袭。
Asian Pac J Cancer Prev. 2013;14(6):3681-4. doi: 10.7314/apjcp.2013.14.6.3681.
10
MicroRNA-376c inhibits cell proliferation and invasion in osteosarcoma by targeting to transforming growth factor-alpha.微小 RNA-376c 通过靶向转化生长因子-α抑制骨肉瘤细胞增殖和侵袭。
DNA Cell Biol. 2013 Jun;32(6):302-9. doi: 10.1089/dna.2013.1977. Epub 2013 Apr 30.
Zotero 插件