Lin Hui-Ju, Chen Wen-Lu, Chen Ter-Hsin, Kung Yung-Jen, Wan Lei
Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan ; School of Chinese Medicine, China Medical University, No.91 Hsueh-shih Road, 404 Taichung, Taiwan.
Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
Biomedicine (Taipei). 2014;4(1):4. doi: 10.7603/s40681-014-0004-3. Epub 2014 Aug 6.
Hypoxia and nitric oxide (NO) play important roles in the onset and progression of glaucoma. Vascular endothelial growth factor (VEGF) is one of the main factors responsive to hypoxia and NO. In this study, we investigated the association between the I C/T VEGF gene polymorphism and primary open angle glaucoma (POAG).
60 POAG patients and 78 healthy volunteers were enrolled in this study. The most frequently observed polymorphism in the VEGF gene is I C/T, which was located 460 nucleotides upstream of the gene. The polymorphism was observed using polymerase chain reaction-based restriction analysis.
Significant differences were observed in the distribution of the polymorphism between control subjects and POAG patients ( = 0.003). C/C homozygotes are absent in the control group; therefore, this genotype represents a suitable genetic maker for POAG.
Hypoxia and NO may be involved in the pathway whereby the VEGF-460 polymorphism regulates POAG. Furthermore, homozygous C/C VEGF genotype is a useful maker for Chinese POAG.
Hypoxia and nitric oxide (NO) play important roles in the onset and progression of glaucoma. Vascular endothelial growth factor (VEGF) is one of the main factors responsive to hypoxia and NO. In this study, we investigated the association between the I C/T VEGF gene polymorphism and primary open angle glaucoma (POAG).
60 POAG patients and 78 healthy volunteers were enrolled in this study. The most frequently observed polymorphism in the VEGF gene is I C/T, which was located 460 nucleotides upstream of the gene. The polymorphism was observed using polymerase chain reaction-based restriction analysis.
Significant differences were observed in the distribution of the polymorphism between control subjects and POAG patients ( = 0.003). C/C homozygotes are absent in the control group; therefore, this genotype represents a suitable genetic maker for POAG.
Hypoxia and NO may be involved in the pathway whereby the VEGF-460 polymorphism regulates POAG. Furthermore, homozygous C/C VEGF genotype is a useful maker for Chinese POAG.
缺氧和一氧化氮（NO）在青光眼的发病和进展中起重要作用。血管内皮生长因子（VEGF）是对缺氧和NO作出反应的主要因子之一。在本研究中，我们调查了VEGF基因IC/T多态性与原发性开角型青光眼（POAG）之间的关联。
本研究纳入了60例POAG患者和78名健康志愿者。VEGF基因中最常观察到的多态性是IC/T，它位于该基因上游460个核苷酸处。使用基于聚合酶链反应的限制性分析来观察该多态性。
在对照组和POAG患者之间观察到该多态性分布存在显著差异（=0.003）。对照组中不存在C/C纯合子；因此，该基因型是POAG的合适遗传标志物。
缺氧和NO可能参与VEGF - 460多态性调节POAG的途径。此外，纯合C/C VEGF基因型是中国POAG的有用标志物。
缺氧和一氧化氮（NO）在青光眼的发病和进展中起重要作用。血管内皮生长因子（VEGF）是对缺氧和NO作出反应的主要因子之一。在本研究中，我们调查了VEGF基因IC/T多态性与原发性开角型青光眼（POAG）之间的关联。
本研究纳入了60例POAG患者和78名健康志愿者。VEGF基因中最常观察到的多态性是IC/T，它位于该基因上游460个核苷酸处。使用基于聚合酶链反应的限制性分析来观察该多态性。
在对照组和POAG患者之间观察到该多态性分布存在显著差异（=0.003）。对照组中不存在C/C纯合子；因此，该基因型是POAG的合适遗传标志物。
缺氧和NO可能参与VEGF - 460多态性调节POAG的途径。此外，纯合C/C VEGF基因型是中国POAG的有用标志物。
