Acute kidney injury and progression of renal failure after fetal programming in the offspring of diabetic rats.
作者信息
Corrêa Rosana R M, Pucci Karla R M, Rocha Laura P, Pereira Júnior Carlos D, Helmo Fernanda R, Machado Juliana R, Rocha Lenaldo B, Rodrigues Aldo R A, Glória Maria A, Guimarães Camila S O, Câmara Niels O S, Reis Marlene A
机构信息
General Pathology Discipline, Department of Genetics, Ecology and General Pathology. Biological and Natural Sciences Institute, Triângulo Mineiro Federal University, Uberaba, Brazil.
Discipline of Physiology, Department of Biochemistry, Molecular Biology, Pharmacology, Physiology and Chemical, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University, Uberaba, Brazil.
出版信息
Pediatr Res. 2015 Mar;77(3):440-6. doi: 10.1038/pr.2014.205. Epub 2014 Dec 18.
BACKGROUND
Diseases of adulthood, such as diabetes and hypertension, may be related to changes during pregnancy, particularly in kidney. We hypothesized that acute kidney injury progresses more rapidly in cases of fetal programming.
METHODS
Diabetic dams' offspring were divided into: CC (controls, receiving vehicle); DC (diabetics, receiving vehicle); CA (controls receiving folic Acid solution, 250 mg/kg); and DA (diabetics receiving folic acid solution). Renal function tests, morphometry, gene, and protein expression of epithelial-mesenchymal transition (EMT) markers were analyzed by qPCR and immunohistochemistry, respectively.
RESULTS
Creatinine, urea, Bowman's space, and EMT markers were increased in CA and DA groups. TGF-β3, actin, and fibronectin expression was higher in CA and DA, with significant increase in DA compared to CA 2-mo offspring. There was higher expression level of TGF-β1, TGF-β3, fibronectin, and vimentin in the offspring of diabetic dams at 5 mo. Increases in TGF-β1 and TGF-β3 were more evident in the offspring of diabetic dams.
CONCLUSION
Fetal programming promotes remarkable changes in kidney morphology, and function in offspring and renal failure progression may be faster in younger offspring of diabetic dams subjected to an additional injury.
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