Dornsife R E, Gasper P W, Mullins J I, Hoover E A
Department of Pathology, Colorado State University, Fort Collins 80523.
Leuk Res. 1989;13(9):745-55. doi: 10.1016/0145-2126(89)90087-8.
Intra-bone marrow inoculation of cells infected with molecularly cloned feline retrovirus (FeLV-C-Sarma [FSC]) associated with aplastic anemia was examined to test the hypothesis that cell-to-cell transmission of virus might facilitate marrow cell infection and anemogenesis, a possibility suggested by in-vitro co-culture experiments. IBM inoculation of either FSC-infected feline marrow cells or fibroblasts of weanling cats bypassed age-related restriction of FSC replication, initiated viremia, caused irreversible depletion of erythroid burst forming units, and induced rapid fatal aplastic anemia. A second significant finding observed with FSC infection was pronounced systemic lymphoid depletion. The direct bone marrow inoculation system described facilitates experimental study of retrovirus-target cell interactions involved in erythroid aplasia.
为了验证病毒的细胞间传播可能会促进骨髓细胞感染和贫血发生这一假说（体外共培养实验提示了这种可能性），研究人员检测了将感染了与再生障碍性贫血相关的分子克隆猫逆转录病毒（FeLV-C-Sarma [FSC]）的细胞进行骨髓内接种的情况。对感染FSC的猫骨髓细胞或断奶小猫的成纤维细胞进行骨髓内接种，绕过了与年龄相关的FSC复制限制，引发了病毒血症，导致红系爆式形成单位不可逆性耗竭，并诱发了快速致命的再生障碍性贫血。FSC感染观察到的另一个重要发现是明显的全身淋巴组织耗竭。所描述的直接骨髓接种系统有助于对涉及红系再生障碍的逆转录病毒-靶细胞相互作用进行实验研究。
