Carnes M, Lent S J, Erisman S, Barksdale C, Feyzi J
Geriatrics Section, Wm. S. Middleton Memorial Veterans Hospital, Madison, WI 53705.
Life Sci. 1989;45(12):1049-56. doi: 10.1016/0024-3205(89)90161-6.
To determine whether CRH is required for the evening rise in plasma ACTH, rats were injected at 0800 hr with CRH antiserum (anti-CRH) or normal rabbit serum (NRS). Blood samples were taken through venous catheters at 0800 hr before treatment and at 1300, 1700, and 2100 hr. Plasma was assayed for immunoreactive ACTH and corticosterone. There was no significant difference in pretreatment values between the two groups. Immunoneutralization of CRH abolished the rise in plasma ACTH seen at 1700 hr in the NRS group but had little effect on earlier levels. The diurnal elevation in plasma corticosterone continued after anti-CRH treatment, but peak levels occurred earlier. Plasma ACTH and corticosterone were significantly correlated at the time of the diurnal surge, but not at 0800 hr or 1300 hr in the NRS controls or at any time point in the anti-CRH group. These results suggest that CRH is required for the diurnal surge of plasma ACTH. They also confirm previous observations by others that the adrenal cortex does not require active CRH or a diurnal surge of ACTH in order to exhibit a significant diurnal increase in secretion of corticosterone, and that factors other than CRH may be relatively more active than CRH in regulation of ACTH secretion during the time of circadian inactivity.
为了确定促肾上腺皮质激素释放激素（CRH）是否是血浆促肾上腺皮质激素（ACTH）夜间升高所必需的，在上午8点给大鼠注射CRH抗血清（抗CRH）或正常兔血清（NRS）。在治疗前的上午8点以及13点、17点和21点通过静脉导管采集血样。检测血浆中的免疫反应性ACTH和皮质酮。两组的预处理值没有显著差异。CRH的免疫中和消除了NRS组在17点时出现的血浆ACTH升高，但对较早时间的水平影响不大。抗CRH治疗后，血浆皮质酮的昼夜升高仍持续，但峰值水平出现得更早。在昼夜高峰时，血浆ACTH和皮质酮显著相关，但在NRS对照组的上午8点或13点以及抗CRH组的任何时间点均不相关。这些结果表明，CRH是血浆ACTH昼夜高峰所必需的。它们还证实了其他人先前的观察结果，即肾上腺皮质不需要活跃的CRH或ACTH的昼夜高峰就能表现出皮质酮分泌的显著昼夜增加，并且在昼夜不活跃期间，除CRH外的其他因素在调节ACTH分泌方面可能比CRH相对更活跃。
