Gill P R, Neilands J B
Department of Biochemistry, University of California, Berkeley 94720.
Mol Microbiol. 1989 Sep;3(9):1183-9. doi: 10.1111/j.1365-2958.1989.tb00268.x.
A collection of transposon-induced mutants of Rhizobium meliloti 1021 defective in siderophore-mediated iron assimilation were obtained and classified as biosynthetic, transport or regulatory. Several of the mutations were cloned and the adjacent sequences were used to acquire complementing DNA from the wild type. A single genomic region of about 35kb complemented all of the mutants deficient in production of the siderophore.
获得了一批苜蓿根瘤菌1021的转座子诱导突变体，这些突变体在铁载体介导的铁同化过程中存在缺陷，并被归类为生物合成型、运输型或调节型。克隆了其中几个突变，并利用相邻序列从野生型中获取互补DNA。一个约35kb的单一基因组区域对所有缺乏铁载体产生能力的突变体都具有互补作用。
