磁共振示踪剂检测神经发生过程中的细胞内 microRNA。
Magnetic resonance beacon to detect intracellular microRNA during neurogenesis.
机构信息
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 270-701, Republic of Korea; Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City 404-834, Republic of Korea.
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 270-701, Republic of Korea; Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City 404-834, Republic of Korea.
出版信息
Biomaterials. 2015 Feb;41:69-78. doi: 10.1016/j.biomaterials.2014.11.033. Epub 2014 Dec 5.
Magnetic resonance imaging (MRI) offers great spatial resolution for viewing deep tissues and anatomy. We developed a self-assembling signal-on magnetic fluorescence nanoparticle to visualize intracellular microRNAs (miRNAs or miRs) during neurogenesis using MRI. The self-assembling nanoparticle (miR124a MR beacon) was aggregated by the incubation of three different oligonucleotides: a 3' adaptor, a 5' adaptor, and a linker containing miR124a-binding sequences. The T2-weighted magnetic resonance (MR) signal of the self-assembled nanoparticle was quenched when miR124a was absent from test tubes or was minimally expressed in cells and tissues. When miR124a was present in test tubes or highly expressed in vitro and in vivo during P19 cell neurogenesis, it hybridized with the miR124a MR beacon, causing the linker to detach, resulting in increased signal-on MRI intensity. This MR beacon can be used as a new imaging probe to monitor the miRNA-mediated regulation of cellular processes.
磁共振成像（MRI）在观察深层组织和解剖结构方面具有很高的空间分辨率。我们开发了一种自组装的信号磁荧光纳米粒子，用于在神经发生过程中使用 MRI 可视化细胞内 microRNAs（miRNAs 或 miR）。自组装纳米粒子（miR124a MR 示踪剂）通过三种不同寡核苷酸的孵育聚集在一起：3' 接头、5' 接头和包含 miR124a 结合序列的连接子。当 miR124a 不存在于试管中或在细胞和组织中表达很少时，自组装纳米粒子的 T2 加权磁共振（MR）信号被猝灭。当 miR124a 存在于试管中或在 P19 细胞神经发生过程中外源高表达时，它与 miR124a MR 示踪剂杂交，导致连接子脱落，从而导致 MRI 信号强度增加。这种 MR 示踪剂可用作新的成像探针，以监测 miRNA 介导的细胞过程调节。
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