钛植入物阴极电压控制电刺激治疗耐甲氧西林金黄色葡萄球菌假体周围感染。
Cathodic voltage-controlled electrical stimulation of titanium implants as treatment for methicillin-resistant Staphylococcus aureus periprosthetic infections.
机构信息
Department of Biomedical Engineering, State University of New York at Buffalo, 162 Farber Hall, 3435 Main Street, Buffalo, NY 14214, USA; Department of Orthopedics, State University of New York at Buffalo, 162 Farber Hall, 3435 Main Street, Buffalo, NY 14214, USA.
Department of Orthopedics, State University of New York at Buffalo, 162 Farber Hall, 3435 Main Street, Buffalo, NY 14214, USA.
出版信息
Biomaterials. 2015 Feb;41:97-105. doi: 10.1016/j.biomaterials.2014.11.013. Epub 2014 Dec 5.
Effective treatment options are often limited for implant-associated orthopedic infections. In this study we evaluated the antimicrobial effects of applying cathodic voltage-controlled electrical stimulation (CVCES) of -1.8 V (vs. Ag/AgCl) to commercially pure titanium (cpTi) substrates with preformed biofilm-like structures of methicillin-resistant Staphylococcus aureus (MRSA). The in vitro studies showed that as compared to the open circuit potential (OCP) conditions, CVCES of -1.8 V for 1 h significantly reduced the colony-forming units (CFU) of MRSA enumerated from the cpTi by 97% (1.89 × 106 vs 6.45 × 104 CFU/ml) and from the surrounding solution by 92% (6.63 × 105 vs. 5.15 × 104 CFU/ml). The in vivo studies, utilizing a rodent periprosthetic infection model, showed that as compared to the OCP conditions, CVCES at -1.8 V for 1 h significantly reduced MRSA CFUs in the bone tissue by 87% (1.15 × 105 vs. 1.48 × 104 CFU/ml) and reduced CFU on the cpTi implant by 98% (5.48 × 104 vs 1.16 × 103 CFU/ml). The stimulation was not associated with histological changes in the host tissue surrounding the implant. As compared to the OCP conditions, the -1.8 V stimulation significantly increased the interfacial capacitance (18.93 vs. 98.25 μF/cm(2)) and decreased polarization resistance (868,250 vs. 108 Ω-cm(2)) of the cpTi. The antimicrobial effects are thought to be associated with these voltage-dependent electrochemical surface properties of the cpTi.
对于与植入物相关的骨科感染，有效的治疗选择通常是有限的。在这项研究中，我们评估了将-1.8 V（相对于 Ag/AgCl）的阴极电压控制电刺激（CVCES）施加到具有耐甲氧西林金黄色葡萄球菌（MRSA）预形成的生物膜样结构的商用纯钛（cpTi）基底上的抗菌效果。体外研究表明，与开路电位（OCP）条件相比，-1.8 V 的 CVCES 处理 1 小时可使从 cpTi 上计数的 MRSA 集落形成单位（CFU）分别减少 97%（1.89×106 与 6.45×104 CFU/ml）和从周围溶液中减少 92%（6.63×105 与 5.15×104 CFU/ml）。利用啮齿动物假体周围感染模型进行的体内研究表明，与 OCP 条件相比，-1.8 V 的 CVCES 处理 1 小时可使骨组织中的 MRSA CFU 减少 87%（1.15×105 与 1.48×104 CFU/ml），并使 cpTi 植入物上的 CFU 减少 98%（5.48×104 与 1.16×103 CFU/ml）。刺激与植入物周围宿主组织的组织学变化无关。与 OCP 条件相比，-1.8 V 刺激可使 cpTi 的界面电容（18.93 与 98.25 μF/cm2）增加 18.93 倍，而极化电阻（868,250 与 108 Ω-cm2）降低 108 倍。抗菌作用被认为与 cpTi 的这些依赖电压的电化学表面特性有关。
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