Young L, Alfieri C, Hennessy K, Evans H, O'Hara C, Anderson K C, Ritz J, Shapiro R S, Rickinson A, Kieff E
Department of Cancer Studies, University of Birmingham, United Kingdom.
N Engl J Med. 1989 Oct 19;321(16):1080-5. doi: 10.1056/NEJM198910193211604.
Epstein-Barr virus (EBV) has been associated with serious or fatal lymphoproliferative disease in immunocompromised patients. EBV nuclear protein 2 and latent membrane protein are characteristically expressed in B lymphocytes proliferating in vitro in response to growth transformation by EBV. These two proteins are thought to be effectors of lymphocyte growth since they increase the expression of B-lymphocyte activation (CD23) and cell-adhesion (LFA 3 and ICAM 1) molecules in vitro. Using monoclonal antibody-immune microscopy, we have demonstrated that these two EBV proteins and their associated B-lymphocyte activation or adhesion molecules are expressed in the infiltrating B lymphocytes in immunocompromised patients with EBV lymphoproliferative disease. These monoclonal antibodies should be useful in the early diagnosis of EBV lymphoproliferative disease and in distinguishing it from other B-lymphocyte cancers associated with EBV, such as Burkitt's lymphoma. The finding of EBV nuclear protein 2 and latent membrane protein and their associated activation or adhesion molecules provides a further pathophysiologic link between EBV and the proliferation of B lymphocytes in immunocompromised patients.
爱泼斯坦-巴尔病毒（EBV）与免疫功能低下患者的严重或致命性淋巴增殖性疾病有关。EBV核蛋白2和潜伏膜蛋白在体外因EBV生长转化而增殖的B淋巴细胞中具有特征性表达。这两种蛋白被认为是淋巴细胞生长的效应因子，因为它们在体外可增加B淋巴细胞活化（CD23）和细胞黏附（淋巴细胞功能相关抗原3和细胞间黏附分子1）分子的表达。利用单克隆抗体免疫显微镜技术，我们已证明这两种EBV蛋白及其相关的B淋巴细胞活化或黏附分子在患有EBV淋巴增殖性疾病的免疫功能低下患者的浸润性B淋巴细胞中表达。这些单克隆抗体在EBV淋巴增殖性疾病的早期诊断以及将其与其他与EBV相关的B淋巴细胞癌（如伯基特淋巴瘤）相鉴别方面应会有所帮助。EBV核蛋白2和潜伏膜蛋白及其相关的活化或黏附分子的发现为EBV与免疫功能低下患者B淋巴细胞增殖之间提供了进一步的病理生理联系。
