Hösli E, Hösli L
Department of Physiology, University of Basle, Switzerland.
Neuroscience. 1989;31(2):463-70. doi: 10.1016/0306-4522(89)90388-6.
The cellular localization of binding sites for [125I] vasoactive intestinal polypeptide and [3H]angiotensin II was studied in explant cultures of rat spinal cord, brain stem, cerebellum and cortex by means of autoradiography. In spinal cord cultures, interneurons of the dorsal horn and motoneurons of the ventral horn were labelled by [125I]vasoactive intestinal polypeptide and [3H]angiotensin II. In many brain stem cultures, groups of large neurons revealed intense binding of both peptides. In contrast, only few medium-sized cerebellar neurons, probably interneurons, showed binding sites for [125I]vasoactive intestinal polypeptide and [3H]angiotensin II. Furthermore, the intensity of labelling of cerebellar neurons was usually weaker than that of neurons of the brain stem and spinal cord. Many neurons in cultures of neocortex were also labelled by [125I]vasoactive intestinal polypeptide, whereas little binding was found with [3H]angiotensin II. In addition to neurons, binding sites for both peptides were also observed on astrocytes. Labelling of these cells was more intense in spinal cord and brain stem cultures than in cultures of cerebellum and cortex, suggesting that only a certain type or a certain population of astrocytes possesses receptors for vasoactive intestinal polypeptide and angiotensin II, or that glial cells in different parts of the CNS have different physiological and pharmacological properties.
通过放射自显影术，研究了[125I]血管活性肠肽和[3H]血管紧张素II结合位点在大鼠脊髓、脑干、小脑和皮质外植体培养物中的细胞定位。在脊髓培养物中，背角中间神经元和腹角运动神经元被[125I]血管活性肠肽和[3H]血管紧张素II标记。在许多脑干培养物中，成群的大神经元显示出两种肽的强烈结合。相比之下，只有少数中等大小的小脑神经元（可能是中间神经元）显示出[125I]血管活性肠肽和[3H]血管紧张素II的结合位点。此外，小脑神经元的标记强度通常比脑干和脊髓神经元的弱。新皮质培养物中的许多神经元也被[125I]血管活性肠肽标记，而[3H]血管紧张素II的结合很少。除神经元外，在星形胶质细胞上也观察到两种肽的结合位点。这些细胞在脊髓和脑干培养物中的标记比在小脑和皮质培养物中更强烈，这表明只有某种类型或特定群体的星形胶质细胞具有血管活性肠肽和血管紧张素II的受体，或者中枢神经系统不同部位的胶质细胞具有不同的生理和药理特性。
