Van Wart-Hood J E, Linder M E, Burr J G
Program in Molecular Biology, University of Texas, Dallas, Richardson 75080.
Oncogene. 1989 Oct;4(10):1267-71.
We have found four compounds that act synergistically with the phosphotyrosine phosphatase inhibitor sodium orthovanadate (Na3VO4) to greatly increase the extent of protein-tyrosine phosphorylation in both uninfected chick embryo fibroblasts (CEF) and their Rous sarcoma virus-transformed counterparts (RSV-CEF). These four inhibitors fall into two categories: the chymotrypsin-specific protease inhibitors, tosyl-phenylalanine-chloromethyl ketone (TPCK) and N-carbobenzoxy-1-phenylalanine-chloromethyl ketone (ZPCK); and certain bioflavonoids which can competitively inhibit ATP-binding, quercetin and phloretin.
我们发现了四种化合物，它们与磷酸酪氨酸磷酸酶抑制剂原钒酸钠（Na3VO4）协同作用，能极大地增加未感染的鸡胚成纤维细胞（CEF）及其劳斯肉瘤病毒转化细胞（RSV-CEF）中蛋白质酪氨酸磷酸化的程度。这四种抑制剂分为两类：胰凝乳蛋白酶特异性蛋白酶抑制剂，甲苯磺酰苯丙氨酸氯甲基酮（TPCK）和N-苄氧羰基-1-苯丙氨酸氯甲基酮（ZPCK）；以及某些能竞争性抑制ATP结合的生物类黄酮，槲皮素和根皮素。
