Welles L, Lopez-Vazquez C M, Hooijmans C M, van Loosdrecht M C M, Brdjanovic D
Department of Environmental Engineering and Water Technology, UNESCO-IHE Institute for Water Education, Westvest 7, 2611AX, Delft, The Netherlands,
Appl Microbiol Biotechnol. 2015 Apr;99(8):3659-72. doi: 10.1007/s00253-014-6287-1. Epub 2014 Dec 19.
The use of saline water in urban areas for non-potable purposes to cope with fresh water scarcity, intrusion of saline water, and disposal of industrial saline wastewater into the sewerage lead to elevated salinity levels in wastewaters. Consequently, saline wastewater is generated, which needs to be treated before its discharge into surface water bodies. The objective of this research was to study the effects of salinity on the aerobic metabolism of phosphate-accumulating organisms (PAO), which belong to the microbial populations responsible for enhanced biological phosphorus removal (EBPR) in activated sludge systems. In this study, the short-term impact (hours) of salinity (as NaCl) was assessed on the aerobic metabolism of a PAO culture, enriched in a sequencing batch reactor (SBR). All aerobic PAO metabolic processes were drastically affected by elevated salinity concentrations. The aerobic maintenance energy requirement increased, when the salinity concentration rose up to a threshold concentration of 2 % salinity (on a W/V basis as NaCl), while above this concentration, the maintenance energy requirements seemed to decrease. All initial rates were affected by salinity, with the NH4- and PO4-uptake rates being the most sensitive. A salinity increase from 0 to 0.18 % caused a 25, 46, and 63 % inhibition of the O2, PO4, and NH4-uptake rates. The stoichiometric ratios of the aerobic conversions confirmed that growth was the process with the highest inhibition, followed by poly-P and glycogen formation. The study indicates that shock loads of 0.18 % salt, which corresponds to the use or intrusion of about 5 % seawater may severely affect the EBPR process already in wastewater treatment plants not exposed regularly to high salinity concentrations.
在城市地区使用盐水作非饮用水用途，以应对淡水短缺、盐水入侵以及将工业含盐废水排入污水管道，导致废水中盐度升高。因此，产生了含盐废水，在其排放到地表水体之前需要进行处理。本研究的目的是研究盐度对聚磷菌（PAO）好氧代谢的影响，聚磷菌属于活性污泥系统中负责强化生物除磷（EBPR）的微生物群体。在本研究中，评估了盐度（以NaCl计）对在序批式反应器（SBR）中富集的PAO培养物好氧代谢的短期影响（数小时）。盐度浓度升高对所有PAO好氧代谢过程都产生了显著影响。当盐度浓度上升至2%盐度（以W/V计，以NaCl计）的阈值浓度时，好氧维持能量需求增加，而高于此浓度时，维持能量需求似乎下降。所有初始速率都受到盐度影响，其中NH4+和PO43-的摄取速率最为敏感。盐度从0增加到0.18%导致O2、PO43-和NH4+摄取速率分别受到25%、46%和63%的抑制。好氧转化的化学计量比证实，生长是受抑制程度最高的过程，其次是聚磷和糖原的形成。该研究表明，0.18%的盐冲击负荷，相当于约5%海水的使用或入侵，可能会严重影响污水处理厂中原本未经常暴露于高盐度浓度下的EBPR工艺。
